Transformation of Penicillium urticae with plasmids containing the hygromycin B resistance gene

A transformation system with efficiencies between 6 and 50 stable transformants per μg of DNA was developed for Penicillium urticae J1 (ATCC48163) using hygromycin B-resistant plasmids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random inte...

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Veröffentlicht in:	Agricultural and biological chemistry 1991-12, Vol.55 (12), p.3053-3057
Hauptverfasser:	Kiuchi, N. (Shinshu Univ., Matsumoto, Nagano (Japan)), Naruse, A, Yamamoto, H, Sekiguchi, J
Format:	Artikel
Sprache:	eng
Schlagworte:	ANTIBIOTICOS ANTIBIOTICS ANTIBIOTIQUE Biotechnology Blotting, Southern Cinnamates DNA - isolation & purification Drug Resistance, Microbial - genetics GENE GENES GENETIC TRANSFORMATION Hygromycin B - analogs & derivatives Hygromycin B - pharmacology PENICILLIUM Penicillium - drug effects Penicillium - genetics PLASMIDE PLASMIDIOS PLASMIDS Protoplasts TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transformation, Genetic
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Beschreibung
Zusammenfassung:	A transformation system with efficiencies between 6 and 50 stable transformants per μg of DNA was developed for Penicillium urticae J1 (ATCC48163) using hygromycin B-resistant plasmids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random integration of vector DNA were observed. Although P. urticae was unable to grow in the presence of 200μg/ml hygromycin B, the transformants were resistant to more than 5 mg/ml of hygromycin B.
ISSN:	0002-1369
DOI:	10.1080/00021369.1991.10860624