Assignment of structures to oligosaccharides produced by enzymic degradation of a β- d-glucan from barley by 1H- and 13C-n.m.r. spectroscopy

The structures of one tri-( 1), two tetra-( 2 and 3), and one hexa-saccharide ( 4) produced by treatment of barley flour, after removal of the starch components, with a fungal β- d-glucanase (Finizyme) have been assigned on the basis of 1H- and 13C-n.m.r. data as follows: β- d-Glc p-(1→3)-β- d-Glc p-(1→4)- d-Glc p ( 1), β- d-Glc p-(1→4)-β- d-Glc p-(1→3)-β- d-Glc p-(1→4)- d-Glc p ( 2), β- d-Glc p-(1→3)-β- d-Glc p-(1→4)-β- d-Glc p-(1→4)- d-Glc p ( 3), and β- d-Xyl p-(1→4)-[α- l-Ara f-(1→3)]-[α- l-Ara f-(1→2)]-β- d-Xyl p-(1→4)-β- d-Xyl p- (1→4)- d-Xyl p ( 4).