Early transitory rise in intracellular pH leads to Bax conformation change during ceramide-induced apoptosis

Early transitory rise in intracellular pH leads to Bax conformation change during ceramide-induced apoptosis

Ceramide can induce apoptosis through a caspase independent pathway. Bax has been described as able to kill cells in the absence of caspase activity, therefore we measured Bax in situ during ceramide-induced apoptosis using anti-Bax antibodies and flow cytometry analysis. An early (2 h) accompanying...

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Veröffentlicht in:Apoptosis (London) 2000-12, Vol.5 (6), p.551-560
Hauptverfasser: Belaud-Rotureau, M A, Leducq, N, Macouillard Poulletier de Gannes, F, Diolez, P, Lacoste, L, Lacombe, F, Bernard, P, Belloc, F
Format: Artikel
Sprache:eng
Schlagworte:
Apoptosis
Apoptosis - drug effects
Apoptosis - physiology
bcl-2-Associated X Protein
Blotting, Western
Cells
Cells, Cultured - drug effects
Cells, Cultured - metabolism
Cellular biology
Ceramides - metabolism
Ceramides - pharmacology
Enzyme Inhibitors - pharmacology
Gene Expression Regulation - drug effects
Gene Expression Regulation - physiology
Humans
Hydrogen-Ion Concentration - drug effects
Intracellular Fluid - drug effects
Intracellular Fluid - metabolism
Intracellular Membranes - drug effects
Intracellular Membranes - metabolism
Intracellular Membranes - ultrastructure
Mitochondria - drug effects
Mitochondria - metabolism
Mitochondria - ultrastructure
Protein Structure, Tertiary - drug effects
Protein Structure, Tertiary - physiology
Proto-Oncogene Proteins - drug effects
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-bcl-2
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Zusammenfassung:Ceramide can induce apoptosis through a caspase independent pathway. Bax has been described as able to kill cells in the absence of caspase activity, therefore we measured Bax in situ during ceramide-induced apoptosis using anti-Bax antibodies and flow cytometry analysis. An early (2 h) accompanying nuclear apoptosis, and was inhibited by Z-VAD-fmk. Bax immuno-reactivity was found to be related to intracellular pH (pHi), and C2-Ceramide (C2-Cer) induced a very early (
ISSN:1360-8185
1573-675X
DOI:10.1023/A:1009693630664