Biliary excretion of hexachloro-1,3-butadiene and its relevance to tissue uptake and renal excretion in male rats

Renal, biliary, pulmonary and faecal excretion experiments were carried out with labelled hexachloro‐1,3‐butadiene ([14C]HCBD) in male Sprague‐Dawley rats, given orally (p.o.) and intravenously (i.v.) in doses of 1 and 100 mg kg−1 as a solution in polyethylene glycol. The radioactivity excreted over 72 h was determined in rats fitted with exteriorized biliary cannulae and in rats whose bile ducts remained fully functional, respectively. In addition, bile duct–duodenum cannula‐linked rats, of which the donor was given 100 mg kg−1 [14C]HCBD orally and the recipient had also a bile fistula, were examined within 30 h for radioactivity in the excreta, the kidney, the liver and the plasma. In non‐cannulated rats, fractional urinary excretion decreased when the dosage increased and amounted to 23% and 8.6% after i.v. injection or 18.5% and 8.9% after p.o. administration of 1 and 100 mg kg−1, respectively. Pulmonary excretion of radioactivity was