Establishment of An Estrogen Receptor-Positive Cell Line (HMA-1) Derived from Human Breast Carcinoma

MATANO, S., OHUCHI, N., HIRAKAWA, H., NISHIHIRA, T., ABE, M., MORI, S. and AKIMOTO, M. Establishment of An Estrogen Receptor-Positive Cell Line (HMA-1) Derived from Human Breast Carcinoma. Tohoku J. Exp. Med., 1991, 164 (2), 169-182 - We have established a novel human breast carcinoma cell line, HMA-1, derived from ascites of a female breast cancer patient. HMA-1 was shown to be an epithelial cell line with intracytoplasmic duct-like vacuoles, microvilli, desmosomes and tonofibrils in accordance with human breast cancer. The cell line demonstrated a good cell growth ability in monolayer fashion with a doubling time of 46hr. Based on a whole cell binding assay the cell line contained estrogen receptor (1.45×10-4 sites/cell). Tamoxifen, an anti-estrogen agent induced a dose-dependent decrease in the cell growth rate, but estradiol stimulated the cell growth. HMA-1 could be transplanted subcutaneously into BALB/c nude mice, and was able to cause tumors approximately two months after heteroinoculation. These results indicate that HMA-1 cell line may serve as a new human breast carcinoma cell line which could be utilized in the breast cancer research.