Development of thermosensitive peptidyl cell substrate synthesis and artificial fibrin glue

A totally synthetic fibrin glue was developed. The molecular structure is a co-polymer of N-isopropyl acrylamide (NIAM) and vinyl monomer units with a cell-adhesion peptidyl moiety (Arg-Gyl-Asp). The buffer solution is a viscous liquid at room temperature, but spontaneous precipitation occurs due to thermoresponsive phase transition derived from poly-NIAM. The peptidyl moieties bind to platelet receptors. When applied to tissues, spontaneous precipitation and subsequent platelet aggregation occurred, indicating that the synthetic bioactive polymer effectively functions like fibrin glue.