DNA-protein cross-linking in L1210 cells sensitive and resistant to cis-diamminedichloroplatinum(II)

The role of restricted cellular accumulation of cis-diamminedichloroplatinum(II) (cis-DDP) and altered repair of DNA-Pt-protein cross-links in the mechanism of L1210 murine leukemia cell resistance was examined. An immunochemical method was used to analyze the formation and removal of DNA-Pt-protein...

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Veröffentlicht in:	Molecular biology reports 1991-05, Vol.15 (2), p.81-86
Hauptverfasser:	OLINSKI, R, BRIGGS, R. C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antineoplastic agents Biological and medical sciences Chromosomal Proteins, Non-Histone - drug effects Chromosomal Proteins, Non-Histone - metabolism Cisplatin - metabolism Cisplatin - pharmacology Cross-Linking Reagents - metabolism Cross-Linking Reagents - pharmacology DNA Damage DNA Repair DNA, Neoplasm - drug effects DNA, Neoplasm - metabolism Drug Resistance General aspects Leukemia L1210 - pathology Medical sciences Mice Neoplasm Proteins - drug effects Neoplasm Proteins - metabolism Pharmacology. Drug treatments
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Zusammenfassung:	The role of restricted cellular accumulation of cis-diamminedichloroplatinum(II) (cis-DDP) and altered repair of DNA-Pt-protein cross-links in the mechanism of L1210 murine leukemia cell resistance was examined. An immunochemical method was used to analyze the formation and removal of DNA-Pt-protein complexes in L1210 cells sensitive and resistant to cis-DDP. The accumulation of Pt into the cells and the binding of Pt to the DNA was measured by atomic absorption spectroscopy. The results demonstrated that both decreased accumulation of the drug and the rate of DNA-Pt protein cross-link removal may be important factors in L1210 cell resistance to cis-DDP.
ISSN:	0301-4851 1573-4978
DOI:	10.1007/BF00364843