Construction of promoter-probe shuttle vectors for Escherichia coli and corynebacteria on the basis of promoterless α-amylase gene

We constructed new promoter-probe vectors for E. coli and corynebacteria based on the promoterless alpha-amylase gene originating from Bacillus subtilis. Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA. alpha-Amylase a...

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Veröffentlicht in:	Folia microbiologica 2000, Vol.45 (2), p.114-120
Hauptverfasser:	UGORCAKOVA, J, BUKOVSKA, G, TIMKO, J
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Amylases - genetics alpha-Amylases - metabolism Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacteriology Biological and medical sciences Corynebacterium - genetics DNA Probes Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genetic Vectors Genetics Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Plasmids - genetics Promoter Regions, Genetic Transformation, Bacterial
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Beschreibung
Zusammenfassung:	We constructed new promoter-probe vectors for E. coli and corynebacteria based on the promoterless alpha-amylase gene originating from Bacillus subtilis. Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA. alpha-Amylase activity can be easily visually detected on agar plates containing a chromogenic substrate, or by direct measurement of alpha-amylase activity.
ISSN:	0015-5632 1874-9356
DOI:	10.1007/BF02817408