Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR

A real-time reverse transcription polymerase chain reaction assay was developed to detect feline calicivirus infection in cats. Using SYBR green I melting curve analysis, isolates could be distinguished by their melting temperature. The assay is sensitive, linear over a wide range of template concen...

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Veröffentlicht in:	Journal of virological methods 2002-12, Vol.106 (2), p.241-244
Hauptverfasser:	Helps, Chris, Lait, Philippa, Tasker, Séverine, Harbour, Dave
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Caliciviridae Infections - diagnosis Caliciviridae Infections - veterinary Caliciviridae Infections - virology Calicivirus, Feline - genetics Calicivirus, Feline - isolation & purification Cat Diseases - diagnosis Cat Diseases - virology Cats FCV Fundamental and applied biological sciences. Psychology Melting curve Microbiology Nucleic Acid Denaturation Real-time PCR Reverse Transcriptase Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - veterinary Techniques used in virology Virology
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Beschreibung
Zusammenfassung:	A real-time reverse transcription polymerase chain reaction assay was developed to detect feline calicivirus infection in cats. Using SYBR green I melting curve analysis, isolates could be distinguished by their melting temperature. The assay is sensitive, linear over a wide range of template concentrations and allows accurate quantitation of viral load.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/S0166-0934(02)00167-2