A split zinc-finger protein is required for normal yeast growth

We have identified a gene that, when present in multiple copies, partially inhibits nuclear protein localization in Saccharomyces cerevisiae. This gene encodes a protein that is a unique member of the Cys 2His 2 zinc-finger family of DNA-binding proteins. It is designated SFP1 for split finger prote...

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Veröffentlicht in:	Gene 1991-10, Vol.107 (1), p.101-110
Hauptverfasser:	Blumberg, Hal, Silver, Pamela
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Base Sequence Blotting, Northern Cloning, Molecular DNA-binding proteins DNA-Binding Proteins - genetics Fluorescent Antibody Technique Fungal Proteins - analysis Fungal Proteins - genetics genbank/m63577 gene cloning gene disruption gene expression Gene Expression - genetics genes Genes, Fungal - genetics growth growth rate high-copy-number library messenger RNA Molecular Sequence Data multiple buds mutagenesis Nuclear protein localization Nuclear Proteins - analysis nucleotide sequence nucleotide sequences polyaspartic acid sequence predictions recombinant DNA Recombinant Fusion Proteins - genetics Restriction Mapping role Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae Proteins sfp1 gene SFP1 protein zinc finger proteins Zinc Fingers - genetics zinc-finger domain
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Zusammenfassung:	We have identified a gene that, when present in multiple copies, partially inhibits nuclear protein localization in Saccharomyces cerevisiae. This gene encodes a protein that is a unique member of the Cys 2His 2 zinc-finger family of DNA-binding proteins. It is designated SFP1 for split finger protein because its two zinc-finger domains are separated from one another by 40 amino acids (aa) as opposed to the usual spacing of 7 or 8 aa for Cys 2His 2 proteins. Disruption of the SFP1 gene results in slow cell growth, with cells having multiple, nucleated buds.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(91)90302-R