Homogeneous solutions of hydrophilic enzymes in nonpolar organic solvents New systems for fundamental studies and biocatalytic transformations

Homogeneous solutions of hydrophilic enzymes in nonpolar organic solvents New systems for fundamental studies and biocatalytic transformations

A typical hydrophilic enzyme, CT, can be dissolved in nonpolar organic solvents ( n-octane, cyclohexane and toluene) up to μM concentrations. In the homogeneous solution obtained, the enzyme possesses catalytic activity and enormously high thermostability. It does not lose this activity even after s...

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Veröffentlicht in:FEBS letters 1991-11, Vol.292 (1), p.159-161
Hauptverfasser: Mozhaev, Vadim V., Poltevsky, Kirill G., Slepnev, Vladimir I., Badun, Gennady A., Levashov, Andrey V.
Format: Artikel
Sprache:eng
Schlagworte:
Activity
alpha -chymotrypsin
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Catalysis
CbzTNE
Cyclohexanes - chemistry
effects on
Enzyme solution
Enzymes - chemistry
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hot Temperature
Hydrolases
N-carbobenoxy-L-tyrosine p-nitrophenyl ester
Octanes - chemistry
Organic solvent
PrOH
propanol-1
Solvents
Stability
Toluene - chemistry
α-chymotrypsin
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Beschreibung
Zusammenfassung:A typical hydrophilic enzyme, CT, can be dissolved in nonpolar organic solvents ( n-octane, cyclohexane and toluene) up to μM concentrations. In the homogeneous solution obtained, the enzyme possesses catalytic activity and enormously high thermostability. It does not lose this activity even after several hours refluxing in octane (126°C) or cyclohexane (81°C).
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(91)80857-Y