Radioimmunoassay of human eosinophil cationic protein (ECP) by an improved method. Establishment of normal levels in serum and turnover in vivo

Summary A radio immunoassay was developed allowing measurement of the cytotoxic cationic ECP. The assay, which has a total incubation time of 3.5 hr, is a double antibody assay with radiolabelled ECP. covering the concentration range of 2–200 μ/l. Performance data show a detection limit of < 2 μg...

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Veröffentlicht in:	Clinical and experimental allergy 1991-09, Vol.21 (5), p.561-567
Hauptverfasser:	PETERSON, C. G. B., ENANDER, I., NYSTRAND, J., ANDERSON, A. S., NILSSON, L., VENGE, P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Blood Proteins - analysis Blood Proteins - metabolism Eosinophil Granule Proteins Eosinophils - metabolism Evaluation Studies as Topic Half-Life Humans Immunological methods for diagnosis and exploration Immunopathology Medical sciences Radioimmunoassay - methods Radioimmunoassay - statistics & numerical data Reference Values Ribonucleases
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Beschreibung
Zusammenfassung:	Summary A radio immunoassay was developed allowing measurement of the cytotoxic cationic ECP. The assay, which has a total incubation time of 3.5 hr, is a double antibody assay with radiolabelled ECP. covering the concentration range of 2–200 μ/l. Performance data show a detection limit of < 2 μg/1 and a cross‐reactivity with eosinophil protein X (EPX/EDN) of
ISSN:	0954-7894 1365-2222
DOI:	10.1111/j.1365-2222.1991.tb00847.x