A simple method for the purification of human peripheral blood monocytes: A substitute for Sepracell-MN

A simple method for the purification of human peripheral blood monocytes: A substitute for Sepracell-MN

Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient wich matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 × 10...

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Veröffentlicht in:Journal of immunological methods 1991-11, Vol.144 (2), p.247-251
Hauptverfasser: Denholm, Elizabeth M., Wolber, Frances M.
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Cell Separation - instrumentation
Cell Separation - methods
Centrifugation, Density Gradient
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Hydrogen-Ion Concentration
Molecular immunology
Monocyte
Monocytes - cytology
Purification
Sepracell
Techniques
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Zusammenfassung:Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient wich matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 × 10 6 monocytes with a purity of 83% were obtained from 30–40 ml of blood. These monocytes were at least 97% viable and responded to chemotactic stimuli in comparable numbers to those prepared using Sepracell-MN.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(91)90092-T