PRODUCTION OF ANTI-PLATELET-ACTIVATING FACTOR ANTIBODIES BY THE USE OF COLLOIDAL GOLD AS CARRIER
Polyclonal and monoclonal antibodies to platelet-activating factor (PAF) were produced, by use of colloidal gold as the hapten carrier. These polyclonal (R88-09) and monoclonal (602 B11) antibodies both reacted with PAF, lysoplatelet-activating factor (lyso PAF), and L-α-lysophosphatidylcholine, pal...
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| Veröffentlicht in: | Japanese Journal of Medical Science and Biology 1991, Vol.44(2), pp.75-80 |
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| container_title | Japanese Journal of Medical Science and Biology |
| container_volume | 44 |
| creator | TOMII, Akiko MASUGI, Fuminori |
| description | Polyclonal and monoclonal antibodies to platelet-activating factor (PAF) were produced, by use of colloidal gold as the hapten carrier. These polyclonal (R88-09) and monoclonal (602 B11) antibodies both reacted with PAF, lysoplatelet-activating factor (lyso PAF), and L-α-lysophosphatidylcholine, palmitoyl (lyso PCP), but did not react with phosphorylcholine chloride (PCC) . Their affinities were higher for PAF than lyso PAF and lyso PCP. |
| doi_str_mv | 10.7883/yoken1952.44.75 |
| format | Article |
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Their affinities were higher for PAF than lyso PAF and lyso PCP.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibody Formation - drug effects</subject><subject>Cross Reactions - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Gold</subject><subject>Haptens - physiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Structure</subject><subject>Platelet Activating Factor - immunology</subject><subject>Rabbits</subject><issn>0021-5112</issn><issn>1884-2828</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1PwkAQxTdGgwQ5ezLZk7fCfpXdHmtboEnDklJMPK3bdqsgX3bhwH9vEYJxDjOT-b15hwfAI0Y9LgTtH7dfZoM9l_QY63H3BrSxEMwhgohb0EaIYMfFmNyDrrVL1NSAcJeJFmg1TwNMRBu8T1MZzoMslhMoh9CfZLEzTfwsSqLM8Zv7q5_FkxEcNrtMf_mLDONoBl_eYDaO4HwWnR4DmSQyDv0EjmQSQn8GAz9N4yh9AHeVXlnTvcwOmA-jLBg7iRzFgZ84BWPe3tG0wrzyOCec5GiQM0qLUuOqQoI1hGDR6FxEPcFomYuSkYHIXY0Z8hjPS5d2wPPZd1dvvw_G7tV6YQuzWumN2R6s4oQJzqnXCPtnYVFvra1NpXb1Yq3ro8JInWJV11gVY4qfrJ8u1od8bco__TnEhodnvrR7_WGuXNf7RbEy__0ujbtXXHzqWpkN_QGf6IKZ</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>TOMII, Akiko</creator><creator>MASUGI, Fuminori</creator><general>National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>PRODUCTION OF ANTI-PLATELET-ACTIVATING FACTOR ANTIBODIES BY THE USE OF COLLOIDAL GOLD AS CARRIER</title><author>TOMII, Akiko ; MASUGI, Fuminori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-a3f17f977272b06b433cda1ff084f17218c445039843db8d4268b5a140947bd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibody Formation - drug effects</topic><topic>Cross Reactions - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Gold</topic><topic>Haptens - physiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Structure</topic><topic>Platelet Activating Factor - immunology</topic><topic>Rabbits</topic><toplevel>online_resources</toplevel><creatorcontrib>TOMII, Akiko</creatorcontrib><creatorcontrib>MASUGI, Fuminori</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese Journal of Medical Science and Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TOMII, Akiko</au><au>MASUGI, Fuminori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PRODUCTION OF ANTI-PLATELET-ACTIVATING FACTOR ANTIBODIES BY THE USE OF COLLOIDAL GOLD AS CARRIER</atitle><jtitle>Japanese Journal of Medical Science and Biology</jtitle><addtitle>JJMSB</addtitle><date>1991</date><risdate>1991</risdate><volume>44</volume><issue>2</issue><spage>75</spage><epage>80</epage><pages>75-80</pages><issn>0021-5112</issn><eissn>1884-2828</eissn><abstract>Polyclonal and monoclonal antibodies to platelet-activating factor (PAF) were produced, by use of colloidal gold as the hapten carrier. 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| fulltext | fulltext |
| identifier | ISSN: 0021-5112 |
| ispartof | Japanese Journal of Medical Science and Biology, 1991, Vol.44(2), pp.75-80 |
| issn | 0021-5112 1884-2828 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72487739 |
| source | MEDLINE; Free E-Journal (出版社公開部分のみ）; J-STAGE |
| subjects | Animals Antibodies, Monoclonal - biosynthesis Antibody Formation - drug effects Cross Reactions - immunology Enzyme-Linked Immunosorbent Assay Female Gold Haptens - physiology Mice Mice, Inbred BALB C Molecular Structure Platelet Activating Factor - immunology Rabbits |
| title | PRODUCTION OF ANTI-PLATELET-ACTIVATING FACTOR ANTIBODIES BY THE USE OF COLLOIDAL GOLD AS CARRIER |
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