PRODUCTION OF ANTI-PLATELET-ACTIVATING FACTOR ANTIBODIES BY THE USE OF COLLOIDAL GOLD AS CARRIER

Polyclonal and monoclonal antibodies to platelet-activating factor (PAF) were produced, by use of colloidal gold as the hapten carrier. These polyclonal (R88-09) and monoclonal (602 B11) antibodies both reacted with PAF, lysoplatelet-activating factor (lyso PAF), and L-α-lysophosphatidylcholine, pal...

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Veröffentlicht in:	Japanese Journal of Medical Science and Biology 1991, Vol.44(2), pp.75-80
Hauptverfasser:	TOMII, Akiko, MASUGI, Fuminori
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - biosynthesis Antibody Formation - drug effects Cross Reactions - immunology Enzyme-Linked Immunosorbent Assay Female Gold Haptens - physiology Mice Mice, Inbred BALB C Molecular Structure Platelet Activating Factor - immunology Rabbits
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Beschreibung
Zusammenfassung:	Polyclonal and monoclonal antibodies to platelet-activating factor (PAF) were produced, by use of colloidal gold as the hapten carrier. These polyclonal (R88-09) and monoclonal (602 B11) antibodies both reacted with PAF, lysoplatelet-activating factor (lyso PAF), and L-α-lysophosphatidylcholine, palmitoyl (lyso PCP), but did not react with phosphorylcholine chloride (PCC) . Their affinities were higher for PAF than lyso PAF and lyso PCP.
ISSN:	0021-5112 1884-2828
DOI:	10.7883/yoken1952.44.75