Application of capillary electrophoresis to the analysis of soluble chromatin

Application of capillary electrophoresis to the analysis of soluble chromatin

Capillary electrophoresis (CE) has been applied to study DNA–protein complexes using as the test system soluble chromatin from chicken erythrocytes and rapidly proliferated cultured Chinese hamster fibroblast‐like cells B11‐dii‐FAF‐28. Separation was performed with home‐made CE apparatus, using a re...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biomedical chromatography 2000-11, Vol.14 (7), p.489-492
Hauptverfasser: Asatiani, Nino V., Abuladze, Marina K., Birkaya, Barbara G., Sapojnikova, Nelly A., Tsibakhashvili, Nelly Ya, Mosulishvili, Liguri M.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Line
Chickens
Chromatin - chemistry
Cricetinae
Cricetulus
Electrophoresis, Capillary - methods
Solubility
Spectrophotometry, Ultraviolet
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Capillary electrophoresis (CE) has been applied to study DNA–protein complexes using as the test system soluble chromatin from chicken erythrocytes and rapidly proliferated cultured Chinese hamster fibroblast‐like cells B11‐dii‐FAF‐28. Separation was performed with home‐made CE apparatus, using a regulated high‐voltage power supply, UV‐detector and fused silica capillaries with inner diameter 75 µm. The heterogeneity of nucleosomal particles with different DNA lengths after micrococcal nuclease digestion was detected. Copyright © 2000 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/1099-0801(200011)14:7<489::AID-BMC998>3.0.CO;2-F