High-speed pectic enzyme fractionation by immobilised metal ion affinity membranes

High-speed pectic enzyme fractionation by immobilised metal ion affinity membranes

Immobilised metal ion affinity polysulfone hollow-fibre membranes, with a high capacity for protein adsorption, were prepared and their utilisation for commercial pectic enzyme fractionation was studied. The pass-through fraction containing pectinlyase is useful for fruit-juice clarification without...

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Veröffentlicht in:Bioseparation 2000, Vol.9 (3), p.173-177
Hauptverfasser: Camperi, S A, Grasselli, M, Cascone, O
Format: Artikel
Sprache:eng
Schlagworte:
Chromatography, Affinity - methods
Copper
Imino Acids
Polygalacturonase - isolation & purification
Polysaccharide-Lyases - isolation & purification
protein purification
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Zusammenfassung:Immobilised metal ion affinity polysulfone hollow-fibre membranes, with a high capacity for protein adsorption, were prepared and their utilisation for commercial pectic enzyme fractionation was studied. The pass-through fraction containing pectinlyase is useful for fruit-juice clarification without methanol production on account of pectinesterase being retained by the IDA-Cu2+ membrane.
ISSN:0923-179X
DOI:10.1023/A:1008187929132