Localization of IpaB Protein in Escherichia coli K-12 MC1061 Strain Carrying Shigella sonnei Form I Plasmid pSS120

We partially purified antigens which reacted with shigellosis convalescent-monkey antisera. Hybridomas, which were constructed from mice immunized by the antigens, produced monoclonal antibodies recognizing IpaB protein. Using the monoclonal antibody against IpaB, evidence indicating that IpaB proteins were localized on the cell surface of invasive bacteria (Escherichia coli K-12 MC1061 harboring pSS120 plasmid) was obtained by immunoelectron microscopy.