Promoter structure and expression of the 3-phosphoglycerate kinase-encoding gene ( pgk1) of Trichoderma reesei
Transcription of the 3-phosphoglycerate kinase (PGK)-encoding gene (pgk1) of Trichoderma reesei results in two transcripts due to two main transcription start points ( tsp) which are differentially regulated during the growth cycle. The nucleotide sequence of the promoter reveals a number of putativ...
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Veröffentlicht in: | Gene 1991-09, Vol.106 (1), p.129-133 |
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Sprache: | eng |
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Zusammenfassung: | Transcription of the 3-phosphoglycerate kinase (PGK)-encoding gene (pgk1) of
Trichoderma reesei results in two transcripts due to two main transcription start points (
tsp) which are differentially regulated during the growth cycle. The nucleotide sequence of the promoter reveals a number of putative regulatory elements present also in the
PGK promoter of
Saccharomyces cerevisiae: a 20-nt long sequence similar to the CTTCC-repeat region of the upstream activating sequence
UAS, the eukaryotic heat-shock consensus sequence,
HSE, and a putative eukaryotic cAMP regulatory sequence. The functionality of the putative
HSE sequence was examined, but no clear effect could be seen on the total amount of
pgk1 mRNA at elevated temperatures nor on transcription initiation from the upstream
tsp, preceded by the
HSE sequence. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(91)90577-X |