Production, characterization and properties of polysaccharide depolymerizing enzymes from a strain of Curvularia inaequalis

Xylanase, beta-glucosidase, beta-xylosidase, endoglucanase and polygalacturonase production from Curvularia inaequalis was carried out by means of solid-state and submerged fermentation using different carbon sources. Beta-glucosidase, beta-xylosidase, polygalacturonase and xylanase produced by the microorganisms were characterized. Beta-glucosidase presented optimum activity at pH 5.5 whereas xylanase, polygalacturonase and beta-xylosidase activities were optimal at pH 5.0. Maximal activity of beta-glucosidase was determined at 60 deg C, beta-xylosidase at 70 deg C, and polygalacturonase and xylanase at 55 deg C. These enzymes were stable at acidic to neutral pH and at 40-45 deg C. The crude enzyme solution was studied for the hydrolysis of agricultural residues.