Chronic ethanol exposure in rats affects rabs-dependent hepatic trafficking of apolipoprotein E and transferrin
Because of the important roles of rabs in protein trafficking, we tested whether chronic ethanol exposure affected the trafficking of newly synthesized apolipoprotein E (apoE) or transferrin ( O-glycosylated and N-glycosylated proteins, respectively) attached to acylated or prenylated rabs. The in v...
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| Veröffentlicht in: | Alcohol (Fayetteville, N.Y.) N.Y.), 2001-11, Vol.25 (3), p.195-200 |
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| creator | Marmillot, Philippe Rao, Manjunath N. Lakshman, M.Raj |
| description | Because of the important roles of rabs in protein trafficking, we tested whether chronic ethanol exposure affected the trafficking of newly synthesized apolipoprotein E (apoE) or transferrin (
O-glycosylated and
N-glycosylated proteins, respectively) attached to acylated or prenylated rabs. The in vivo 30-min incorporation ratios of [
3H]palmitate:[
35S]methionine or [
3H]mevalonate:[
35S]methionine (relative ratios of rabs acylation or prenylation to total protein or to immunoisolated apoE or transferrin) were measured in various hepatic subcellular organelles of 8 week-ethanol-fed (E) and pair-fed control (C) Wistar–Furth rats. With respect to total protein trafficking, ethanol increased rabs acylation ratio by 136% (
P <
.01), 69% (
P < .05), and 64% (
P < .01) in the endoplasmic reticulum (ER), Golgi light fraction (GLF), and Golgi heavy fraction (GHF), respectively, and decreased this ratio by 76% (
P < .01) in carrier vesicle fraction 2 (CV2). With respect to apoE trafficking, ethanol increased rabs acylation ratio by 121% in GHF and decreased this ratio by 27% in CV2. Rabs prenylation ratio increased by 21% and 53% in GHF and CV2, respectively, and decreased by 42% in GLF. With respect to transferrin trafficking, ethanol increased rabs acylation ratio by 53% (
P < .01) in GHF, with no significant effect in ER, whereas rabs prenylation ratio increased by 26% (
P < .05) in ER, with no significant effect in GHF. Therefore, we conclude that ethanol-induced impaired trafficking of newly synthesized
O- and
N-glycosylated proteins occurs primarily in ER and Golgi and is due to altered lipidation of rabs, possibly rabs 1, 2, or 6 or combinations of these three rabs. |
| doi_str_mv | 10.1016/S0741-8329(01)00179-3 |
| format | Article |
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O-glycosylated and
N-glycosylated proteins, respectively) attached to acylated or prenylated rabs. The in vivo 30-min incorporation ratios of [
3H]palmitate:[
35S]methionine or [
3H]mevalonate:[
35S]methionine (relative ratios of rabs acylation or prenylation to total protein or to immunoisolated apoE or transferrin) were measured in various hepatic subcellular organelles of 8 week-ethanol-fed (E) and pair-fed control (C) Wistar–Furth rats. With respect to total protein trafficking, ethanol increased rabs acylation ratio by 136% (
P <
.01), 69% (
P < .05), and 64% (
P < .01) in the endoplasmic reticulum (ER), Golgi light fraction (GLF), and Golgi heavy fraction (GHF), respectively, and decreased this ratio by 76% (
P < .01) in carrier vesicle fraction 2 (CV2). With respect to apoE trafficking, ethanol increased rabs acylation ratio by 121% in GHF and decreased this ratio by 27% in CV2. Rabs prenylation ratio increased by 21% and 53% in GHF and CV2, respectively, and decreased by 42% in GLF. With respect to transferrin trafficking, ethanol increased rabs acylation ratio by 53% (
P < .01) in GHF, with no significant effect in ER, whereas rabs prenylation ratio increased by 26% (
P < .05) in ER, with no significant effect in GHF. Therefore, we conclude that ethanol-induced impaired trafficking of newly synthesized
O- and
N-glycosylated proteins occurs primarily in ER and Golgi and is due to altered lipidation of rabs, possibly rabs 1, 2, or 6 or combinations of these three rabs.]]></description><identifier>ISSN: 0741-8329</identifier><identifier>EISSN: 1873-6823</identifier><identifier>DOI: 10.1016/S0741-8329(01)00179-3</identifier><identifier>PMID: 11839466</identifier><identifier>CODEN: ALCOEX</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Alcoholism ; Alcoholism and acute alcohol poisoning ; Animals ; ApoE ; Apolipoproteins E - metabolism ; Biological and medical sciences ; Central Nervous System Depressants - pharmacology ; Endoplasmic Reticulum - drug effects ; Endoplasmic Reticulum - metabolism ; Ethanol - pharmacology ; Golgi Apparatus - drug effects ; Golgi Apparatus - metabolism ; Intracellular Fluid - metabolism ; Liver - drug effects ; Liver - metabolism ; Male ; Medical sciences ; Protein trafficking ; Protein Transport - drug effects ; Protein Transport - physiology ; rab GTP-Binding Proteins - physiology ; Rabs ; Rats ; Rats, Inbred WF ; Toxicology ; Transferrin ; Transferrin - metabolism</subject><ispartof>Alcohol (Fayetteville, N.Y.), 2001-11, Vol.25 (3), p.195-200</ispartof><rights>2002 Elsevier Science Inc.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-b7d0a89a7525ce01422ad0be20b9d725c93e84eb8cfa932173e9673bc60f8c0c3</citedby><cites>FETCH-LOGICAL-c422t-b7d0a89a7525ce01422ad0be20b9d725c93e84eb8cfa932173e9673bc60f8c0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0741-8329(01)00179-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3541,23921,23922,25131,27915,27916,45986,64376</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13509995$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11839466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marmillot, Philippe</creatorcontrib><creatorcontrib>Rao, Manjunath N.</creatorcontrib><creatorcontrib>Lakshman, M.Raj</creatorcontrib><title>Chronic ethanol exposure in rats affects rabs-dependent hepatic trafficking of apolipoprotein E and transferrin</title><title>Alcohol (Fayetteville, N.Y.)</title><addtitle>Alcohol</addtitle><description><![CDATA[Because of the important roles of rabs in protein trafficking, we tested whether chronic ethanol exposure affected the trafficking of newly synthesized apolipoprotein E (apoE) or transferrin (
O-glycosylated and
N-glycosylated proteins, respectively) attached to acylated or prenylated rabs. The in vivo 30-min incorporation ratios of [
3H]palmitate:[
35S]methionine or [
3H]mevalonate:[
35S]methionine (relative ratios of rabs acylation or prenylation to total protein or to immunoisolated apoE or transferrin) were measured in various hepatic subcellular organelles of 8 week-ethanol-fed (E) and pair-fed control (C) Wistar–Furth rats. With respect to total protein trafficking, ethanol increased rabs acylation ratio by 136% (
P <
.01), 69% (
P < .05), and 64% (
P < .01) in the endoplasmic reticulum (ER), Golgi light fraction (GLF), and Golgi heavy fraction (GHF), respectively, and decreased this ratio by 76% (
P < .01) in carrier vesicle fraction 2 (CV2). With respect to apoE trafficking, ethanol increased rabs acylation ratio by 121% in GHF and decreased this ratio by 27% in CV2. Rabs prenylation ratio increased by 21% and 53% in GHF and CV2, respectively, and decreased by 42% in GLF. With respect to transferrin trafficking, ethanol increased rabs acylation ratio by 53% (
P < .01) in GHF, with no significant effect in ER, whereas rabs prenylation ratio increased by 26% (
P < .05) in ER, with no significant effect in GHF. Therefore, we conclude that ethanol-induced impaired trafficking of newly synthesized
O- and
N-glycosylated proteins occurs primarily in ER and Golgi and is due to altered lipidation of rabs, possibly rabs 1, 2, or 6 or combinations of these three rabs.]]></description><subject>Alcoholism</subject><subject>Alcoholism and acute alcohol poisoning</subject><subject>Animals</subject><subject>ApoE</subject><subject>Apolipoproteins E - metabolism</subject><subject>Biological and medical sciences</subject><subject>Central Nervous System Depressants - pharmacology</subject><subject>Endoplasmic Reticulum - drug effects</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Ethanol - pharmacology</subject><subject>Golgi Apparatus - drug effects</subject><subject>Golgi Apparatus - metabolism</subject><subject>Intracellular Fluid - metabolism</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Protein trafficking</subject><subject>Protein Transport - drug effects</subject><subject>Protein Transport - physiology</subject><subject>rab GTP-Binding Proteins - physiology</subject><subject>Rabs</subject><subject>Rats</subject><subject>Rats, Inbred WF</subject><subject>Toxicology</subject><subject>Transferrin</subject><subject>Transferrin - metabolism</subject><issn>0741-8329</issn><issn>1873-6823</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS1ERZfCTwD5QkUPKWM7ieMTQqtSKlXqAThbjj1mDVk72FlE_z3e7ooeexpp9L2Zp_cIecPgkgHrP3wF2bJmEFy9B3YBwKRqxDOyYoMUTT9w8Zys_iOn5GUpPwFASqlekFPGBqHavl-RtN7kFIOluGxMTBPFv3Mqu4w0RJrNUqjxHm2d2YylcThjdBgXusHZLFW35AoE-yvEHzR5auY0hTnNOS1YL1xRE92eicVjziG-IifeTAVfH-cZ-f756tv6S3N7d32z_nTb2JbzpRmlAzMoIzveWQRWl8bBiBxG5WTdKYFDi-NgvVGCMylQ9VKMtgc_WLDijJwf7lYnv3dYFr0NxeI0mYhpV7TkLZfQd0-CNSngSskKdgfQ5lRKRq_nHLYm32sGel-JfqhE7_PWwPRDJVpU3dvjg924RfeoOnZQgXdHwBRrJl_DsqE8cqIDpdTe6ccDhzW3PwGzLjZgtOhCrg1pl8ITVv4B-iSp7Q</recordid><startdate>20011101</startdate><enddate>20011101</enddate><creator>Marmillot, Philippe</creator><creator>Rao, Manjunath N.</creator><creator>Lakshman, M.Raj</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20011101</creationdate><title>Chronic ethanol exposure in rats affects rabs-dependent hepatic trafficking of apolipoprotein E and transferrin</title><author>Marmillot, Philippe ; Rao, Manjunath N. ; Lakshman, M.Raj</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-b7d0a89a7525ce01422ad0be20b9d725c93e84eb8cfa932173e9673bc60f8c0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Alcoholism</topic><topic>Alcoholism and acute alcohol poisoning</topic><topic>Animals</topic><topic>ApoE</topic><topic>Apolipoproteins E - metabolism</topic><topic>Biological and medical sciences</topic><topic>Central Nervous System Depressants - pharmacology</topic><topic>Endoplasmic Reticulum - drug effects</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Ethanol - pharmacology</topic><topic>Golgi Apparatus - drug effects</topic><topic>Golgi Apparatus - metabolism</topic><topic>Intracellular Fluid - metabolism</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Protein trafficking</topic><topic>Protein Transport - drug effects</topic><topic>Protein Transport - physiology</topic><topic>rab GTP-Binding Proteins - physiology</topic><topic>Rabs</topic><topic>Rats</topic><topic>Rats, Inbred WF</topic><topic>Toxicology</topic><topic>Transferrin</topic><topic>Transferrin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marmillot, Philippe</creatorcontrib><creatorcontrib>Rao, Manjunath N.</creatorcontrib><creatorcontrib>Lakshman, M.Raj</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Alcohol (Fayetteville, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marmillot, Philippe</au><au>Rao, Manjunath N.</au><au>Lakshman, M.Raj</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chronic ethanol exposure in rats affects rabs-dependent hepatic trafficking of apolipoprotein E and transferrin</atitle><jtitle>Alcohol (Fayetteville, N.Y.)</jtitle><addtitle>Alcohol</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>25</volume><issue>3</issue><spage>195</spage><epage>200</epage><pages>195-200</pages><issn>0741-8329</issn><eissn>1873-6823</eissn><coden>ALCOEX</coden><abstract><![CDATA[Because of the important roles of rabs in protein trafficking, we tested whether chronic ethanol exposure affected the trafficking of newly synthesized apolipoprotein E (apoE) or transferrin (
O-glycosylated and
N-glycosylated proteins, respectively) attached to acylated or prenylated rabs. The in vivo 30-min incorporation ratios of [
3H]palmitate:[
35S]methionine or [
3H]mevalonate:[
35S]methionine (relative ratios of rabs acylation or prenylation to total protein or to immunoisolated apoE or transferrin) were measured in various hepatic subcellular organelles of 8 week-ethanol-fed (E) and pair-fed control (C) Wistar–Furth rats. With respect to total protein trafficking, ethanol increased rabs acylation ratio by 136% (
P <
.01), 69% (
P < .05), and 64% (
P < .01) in the endoplasmic reticulum (ER), Golgi light fraction (GLF), and Golgi heavy fraction (GHF), respectively, and decreased this ratio by 76% (
P < .01) in carrier vesicle fraction 2 (CV2). With respect to apoE trafficking, ethanol increased rabs acylation ratio by 121% in GHF and decreased this ratio by 27% in CV2. Rabs prenylation ratio increased by 21% and 53% in GHF and CV2, respectively, and decreased by 42% in GLF. With respect to transferrin trafficking, ethanol increased rabs acylation ratio by 53% (
P < .01) in GHF, with no significant effect in ER, whereas rabs prenylation ratio increased by 26% (
P < .05) in ER, with no significant effect in GHF. Therefore, we conclude that ethanol-induced impaired trafficking of newly synthesized
O- and
N-glycosylated proteins occurs primarily in ER and Golgi and is due to altered lipidation of rabs, possibly rabs 1, 2, or 6 or combinations of these three rabs.]]></abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>11839466</pmid><doi>10.1016/S0741-8329(01)00179-3</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Alcohol (Fayetteville, N.Y.), 2001-11, Vol.25 (3), p.195-200 |
| issn | 0741-8329 1873-6823 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); ProQuest Central UK/Ireland |
| subjects | Alcoholism Alcoholism and acute alcohol poisoning Animals ApoE Apolipoproteins E - metabolism Biological and medical sciences Central Nervous System Depressants - pharmacology Endoplasmic Reticulum - drug effects Endoplasmic Reticulum - metabolism Ethanol - pharmacology Golgi Apparatus - drug effects Golgi Apparatus - metabolism Intracellular Fluid - metabolism Liver - drug effects Liver - metabolism Male Medical sciences Protein trafficking Protein Transport - drug effects Protein Transport - physiology rab GTP-Binding Proteins - physiology Rabs Rats Rats, Inbred WF Toxicology Transferrin Transferrin - metabolism |
| title | Chronic ethanol exposure in rats affects rabs-dependent hepatic trafficking of apolipoprotein E and transferrin |
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