Preparation of Symmetrical and Unsymmetrical DNA-Protein Conjugates with DNA as a Molecular Scaffold

Preparation of Symmetrical and Unsymmetrical DNA-Protein Conjugates with DNA as a Molecular Scaffold

DNA “dumbbells” (shown schematically) with either the same or different proteins attached to the ends of DNA oligonucleotides were prepared by using the polymerase chain reaction to introduce the points of attachment (Y and W), and imaged by atomic force microscopy. In some cases, the observed value...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chembiochem : a European journal of chemical biology 2001-05, Vol.2 (5), p.375-378
Hauptverfasser: Tomkins, Justin M., Nabbs, Brent K., Barnes, Karen, Legido, Marta, Blacker, A. John, McKendry, Rachel A., Abell, Chris
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Biotinylation
Cross-Linking Reagents
DNA - chemistry
DNA structures
Macromolecular Substances
Microscopy, Atomic Force
Molecular Probes - chemical synthesis
Molecular Probes - chemistry
nanostructures
Nanotechnology
Oligodeoxyribonucleotides - chemistry
oligonucleotides
Proteins - chemistry
scanning probe microscopy
single molecules
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:DNA “dumbbells” (shown schematically) with either the same or different proteins attached to the ends of DNA oligonucleotides were prepared by using the polymerase chain reaction to introduce the points of attachment (Y and W), and imaged by atomic force microscopy. In some cases, the observed values for the distance separation between the two proteins were smaller than the calculated ones, which is most probably due to bending of the scaffold DNA.
ISSN:1439-4227
1439-7633
DOI:10.1002/1439-7633(20010504)2:5<375::AID-CBIC375>3.0.CO;2-I