A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae
A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive co...
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| Veröffentlicht in: | Leprosy review 2001-12, Vol.72 (4), p.441-448 |
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| container_title | Leprosy review |
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| creator | Honoré, N Roche, P W Grosset, J H Cole, S T |
| description | A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different non-radioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients. |
| doi_str_mv | 10.5935/0305-7518.20010052 |
| format | Article |
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The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Drug Resistance, Microbial</subject><subject>Humans</subject><subject>Leprostatic Agents - pharmacology</subject><subject>Leprosy - diagnosis</subject><subject>Leprosy - drug therapy</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Mycobacterium leprae - drug effects</subject><subject>Mycobacterium leprae - genetics</subject><subject>Mycobacterium leprae - isolation & purification</subject><subject>Oligonucleotide Probes</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - standards</subject><subject>Predictive Value of Tests</subject><subject>Rifampin - pharmacology</subject><issn>0305-7518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkDtPwzAYRT2AaCn8AQbkiS3FjuMmHquKl1TEQmfLj8_CKImD7Qz99ySiwHSlq3PvcBC6oWTNBeP3hBFe1Jw265IQSggvz9Dyr1ygy5Q-CWGUCnaBFpQ25aZqyBIdtriD_BEsdiHiqAZvsYUMJvvQ4-Bw9E51gze-LyIkn7LqM_YptCpDmoHXowlamQzRjx1uYYgKrtC5U22C61Ou0OHx4X33XOzfnl52231hSiFywTnfMO5qwwm3ihJT1dRqq5uGOUWtcAJMI5zmum4mtKqVpVQTI7RlptbAVuju53eI4WuElGXnk4G2VT2EMcm6rCYd1WYCyx_QxJBSBCeH6DsVj5ISOQuUsys5u5K_AqfR7el91B3Y_8nJHvsG81Nuaw</recordid><startdate>20011201</startdate><enddate>20011201</enddate><creator>Honoré, N</creator><creator>Roche, P W</creator><creator>Grosset, J H</creator><creator>Cole, S T</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011201</creationdate><title>A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae</title><author>Honoré, N ; Roche, P W ; Grosset, J H ; Cole, S T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c299t-555635f7c505da10c471dbdb883fa1d9f9ec89fb5b7855647ad11b0c9bd3c7be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Drug Resistance, Microbial</topic><topic>Humans</topic><topic>Leprostatic Agents - pharmacology</topic><topic>Leprosy - diagnosis</topic><topic>Leprosy - drug therapy</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Mycobacterium leprae - drug effects</topic><topic>Mycobacterium leprae - genetics</topic><topic>Mycobacterium leprae - isolation & purification</topic><topic>Oligonucleotide Probes</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - standards</topic><topic>Predictive Value of Tests</topic><topic>Rifampin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Honoré, N</creatorcontrib><creatorcontrib>Roche, P W</creatorcontrib><creatorcontrib>Grosset, J H</creatorcontrib><creatorcontrib>Cole, S T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Leprosy review</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Honoré, N</au><au>Roche, P W</au><au>Grosset, J H</au><au>Cole, S T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae</atitle><jtitle>Leprosy review</jtitle><addtitle>Lepr Rev</addtitle><date>2001-12-01</date><risdate>2001</risdate><volume>72</volume><issue>4</issue><spage>441</spage><epage>448</epage><pages>441-448</pages><issn>0305-7518</issn><abstract>A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. 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| fulltext | fulltext |
| identifier | ISSN: 0305-7518 |
| ispartof | Leprosy review, 2001-12, Vol.72 (4), p.441-448 |
| issn | 0305-7518 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72420046 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Base Sequence Drug Resistance, Microbial Humans Leprostatic Agents - pharmacology Leprosy - diagnosis Leprosy - drug therapy Mice Molecular Sequence Data Mycobacterium leprae - drug effects Mycobacterium leprae - genetics Mycobacterium leprae - isolation & purification Oligonucleotide Probes Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Predictive Value of Tests Rifampin - pharmacology |
| title | A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae |
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