A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae

A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive co...

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Veröffentlicht in:	Leprosy review 2001-12, Vol.72 (4), p.441-448
Hauptverfasser:	Honoré, N, Roche, P W, Grosset, J H, Cole, S T
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Drug Resistance, Microbial Humans Leprostatic Agents - pharmacology Leprosy - diagnosis Leprosy - drug therapy Mice Molecular Sequence Data Mycobacterium leprae - drug effects Mycobacterium leprae - genetics Mycobacterium leprae - isolation & purification Oligonucleotide Probes Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Predictive Value of Tests Rifampin - pharmacology
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Beschreibung
Zusammenfassung:	A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different non-radioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.
ISSN:	0305-7518
DOI:	10.5935/0305-7518.20010052