Improving the performance of an acid-labile 4-hydroxymethyl phenoxyacetic acid (HMP) linker on resin and SynPhase™ grafted solid-supports

A replacement of the acetic acid moiety by valeric acid within the 4‐hydroxymethylphenoxyacetic acid (HMP) linker (Sheppard RC, Williams BJ. Acid‐labile resin linkage agents for use in solid phase peptide synthesis. Int. J. Peptide Protein Res. 1982; 20: 451–454) significantly improved its performance in terms of loading capacity, yield and purity of the final products. The results indicated the spacer–linker combination and type of solid supports are important factors for solid‐phase synthesis. Copyright © 2000 European Peptide Society and John Wiley & Sons, Ltd.