A new developmentally regulated gene family in Leishmania amastigotes encoding a homolog of amastin surface proteins

The ability of Leishmania to survive within the phagolysosomes of mammalian macrophages is heavily dependent on the developmental regulation of a number of genes. Characterization of genes preferentially expressed during the parasite's intracellular growth would help to elucidate the mechanisms controlling stage-specific gene regulation and the intracellular life of the parasite in general. Using a genomic approach based on the differential hybridization screening of high-density filters, we have identified a new developmentally regulated gene in Leishmania, which is part of a multigene family and encodes a highly hydrophobic protein that shares homology with the Trypanosoma cruzi amastin proteins . The fusion of the Leishmania amastin gene homolog with the green fluorescent protein and analysis by confocal microscopy suggested a surface localization for this protein. The amastin gene homolog is expressed predominantly in the amastigote form of several Leishmania species and is strictly regulated by acidic pH at the post-transcriptional level. Its developmental expression involves sequences within the 3′-untranslated region.