Proteomics in the post-genome age

The genome sequencing effort has helped spawn the burgeoning field of proteomics. This review article examines state‐of‐the‐art proteomics methods that are helping change the discovery paradigm in a variety of biological disciplines and, in particular, protein biochemistry. The review discusses both classical and novel methods to perform high‐throughput qualitative and quantitative “global” as well as targeted proteome analysis of complex biological systems. From a drug discovery standpoint, the synergy between genomics and proteomics will help elucidate disease mechanisms, identify novel drug targets, and identify surrogate biomarkers that could be used to conduct clinical trials. © 2001 John Wiley & Sons, Inc. Biopolymers (Pept Sci) 60: 206–211, 2001