Helicobacter pylori cag pathogenicity island is associated with enhanced Interleukin-8 expression in human gastric mucosa

Background. In vitro studies showed that Helicobacter pylori strains carrying the cag pathogenicity island are able to induce epithelial secretion of Interleukin-8. Aims. To evaluate the assessment of cag pathogenicity island and the expression of Interleukin-8 in the gastric mucosa of Helicobacter pylori-infected patients and correlate these data with the activity of gastritis and Helicobacter pylori density. Methods. cag status was determined by polymerase chain reaction directly on gastric biopsies from 13 Helicobacter pylori+ patients with non-ulcer dyspepsia and 13 Helicobacter pylori+ with duodenal ulcer. Interleukin-8 gene transcription and protein expression were analysed by in situ hybridization and immunofluorescence, respectively. Gastritis activity and Helicobacter pylori density were also investigated. Results. cag was present in 20 26 of Helicobacter pylori+ patients: in 7 13 non-ulcer dyspepsia (53.8%) and in 13 13 duodenal ulcer patients (100%), (p