A DNA Microarray-Based Genetic Screen for Nonhomologous End-Joining Mutants in Saccharomyces cerevisiae

We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in the yeast genome. The presence or absence of mutants was detected by oligonucleotide arrays that hybridize to 20-nucleotide "barcodes.&q...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2001-12, Vol.294 (5551), p.2552-2556
Hauptverfasser:	Ooi, Siew Loon, Shoemaker, Daniel D., Boeke, Jef D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences CCAAT-Binding Factor - genetics CCAAT-Binding Factor - metabolism Diploidy DNA DNA Ligase ATP DNA Ligases - genetics DNA Ligases - metabolism DNA microarrays DNA probes DNA Repair DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - metabolism Genes Genes, Fungal Genetic aspects Genetic Complementation Test Genetic screening Genetic testing Genetics Genomics Haploidy Legends Molecular and cellular biology Molecular biology Mutation NEJ1 gene Nej1 protein Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Open reading frames Plasmids Recombination, Genetic Saccharomyces Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Transcription Factors - genetics Transcription Factors - metabolism Transformation, Genetic Two-Hybrid System Techniques Yeast Yeasts
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Zusammenfassung:	We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in the yeast genome. The presence or absence of mutants was detected by oligonucleotide arrays that hybridize to 20-nucleotide "barcodes." We used this method to screen for components of the nonhomologous end-joining (NHEJ) pathway. Known components of the pathway were identified, as well as a gene not previously known to be involved in NHEJ, NEJ1. Nej1 protein interacts with the amino terminus of LIF1/XRCC4, a recently recognized "guardian of the genome" against cancer.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.1065672