Purification, crystallization and preliminary diffraction study of β-galactosidase from Penicillium sp

Purification, crystallization and preliminary diffraction study of β-galactosidase from Penicillium sp

Crystals of an extracellular β‐galactosidase from Penicillium sp. (MW = 120 ± 5 kDa) have been obtained from a sodium phosphate buffer using PEG as precipitant. The crystals belong to the tetragonal space group P41or P43, with unit‐cell parameters a = b = 110.82, c = 161.28 Å, and diffract to 1.85 Å...

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Veröffentlicht in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2000-11, Vol.56 (11), p.1508-1509
Hauptverfasser: Neustroev, K. N., De Sousa, E. A., Golubev, A. M., Brandão Neto, J. R., Eneyskaya, E. V., Kulminskaya, A. A., Polikarpov, I.
Format: Artikel
Sprache:eng
Schlagworte:
beta-Galactosidase - chemistry
beta-Galactosidase - isolation & purification
beta-Galactosidase - metabolism
Crystallization
Crystallography, X-Ray
Glycosylation
Penicillium - enzymology
Protein Conformation
β-galactosidase
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Zusammenfassung:Crystals of an extracellular β‐galactosidase from Penicillium sp. (MW = 120 ± 5 kDa) have been obtained from a sodium phosphate buffer using PEG as precipitant. The crystals belong to the tetragonal space group P41or P43, with unit‐cell parameters a = b = 110.82, c = 161.28 Å, and diffract to 1.85 Å resolution at a synchrotron source.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444900011756