Dantrolene ameliorates delayed cell death and concomitant DNA fragmentation in the rat hippocampal CA1 neurons subjected to mild ischemia

The present study investigated whether dantrolene, which inhibits the Ca 2+ release from the intracellular Ca 2+ store sites, reduced nuclear DNA fragmentation and produced neuronal protection in a model of global forebrain ischemia. Male Wistar rats were subjected to four-vessel occlusion (4VO) for...

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Veröffentlicht in:Resuscitation 2001-07, Vol.50 (1), p.117-125
Hauptverfasser: Yano, Toshiyuki, Nakayama, Ryosuke, Imaizumi, Takashi, Terasaki, Hidenori, Ushijima, Kazuo
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Sprache:eng
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Zusammenfassung:The present study investigated whether dantrolene, which inhibits the Ca 2+ release from the intracellular Ca 2+ store sites, reduced nuclear DNA fragmentation and produced neuronal protection in a model of global forebrain ischemia. Male Wistar rats were subjected to four-vessel occlusion (4VO) for 5 min and then infused continuously with dantrolene or vehicle into the cerebral ventricle for 3 days. The intact rats did not undergo any intervention. The number of viable and DNA nick-end-labeled neurons in the hippocampal CA1 were evaluated 4 days after the ischemia. The number of viable neurons in the dantrolene-treated rats was significantly higher than that in the vehicle-treated rats and lower than that in the intact animals ( P
ISSN:0300-9572
1873-1570
DOI:10.1016/S0300-9572(00)00369-5