Choriocarcinoma-like human chorionic gonadotrophin (HCG) and HCG bioactivity during the first trimester of pregnancy
The objective of this study was to evaluate the distribution of choriocarcinoma-like human chorionic gonadotrophin (HCG) isoforms during first trimester pregnancy and their relationship with in-vitro HCG bioactivity. This was done by means of a retrospective analysis of patients' sera with firs...
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| Veröffentlicht in: | Human reproduction (Oxford) 2000-10, Vol.15 (10), p.2209-2214 |
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| creator | Mock, Pascal Kovalevskaya, Galina O'Connor, John F. Campana, Aldo |
| description | The objective of this study was to evaluate the distribution of choriocarcinoma-like human chorionic gonadotrophin (HCG) isoforms during first trimester pregnancy and their relationship with in-vitro HCG bioactivity. This was done by means of a retrospective analysis of patients' sera with first trimester normal intrauterine and abnormal (ectopic) pregnancies. Serum samples were obtained from 38 women with an amenorrhoea of |
| doi_str_mv | 10.1093/humrep/15.10.2209 |
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This was done by means of a retrospective analysis of patients' sera with first trimester normal intrauterine and abnormal (ectopic) pregnancies. Serum samples were obtained from 38 women with an amenorrhoea of <10 weeks. From these, 19 had a normal intrauterine pregnancy (IUP) and 19 an ectopic pregnancy (EP). Total immunoreactive HCG (HCGi), free β-HCGi and oestradiol were measured by enzyme immunoassays and bioactive HCG by the mouse Leydig cell bioassay. The alterations in HCG isoform content were measured by the combination of two immunometric assays, B152 for choriocarcinoma-like HCG and B109 for intact HCG detection and expressed as the B152/B109 ratio. Choriocarcinoma-like HCG isoforms ratio measured by B152 and B109 assays was significantly higher in the low subgroups of free β-HCGi and gestational age (P = 0.0111 and 0.0036 respectively). Whereas bioactive to immunoreactive HCG ratios (b/i ratio) were significantly higher when free β-HCGi concentrations were low (P = 0.0010), no correlation was found between the variation of bioactivity (b/i ratio) and the proportion of choriocarcinoma-like HCG isoforms (B159/B108). It is concluded that in first trimester pregnancies (i) the modulation of HCG in-vitro bioactivity is not related to the variation of choriocarcinoma-like HCG isoforms secretion and (ii) the amount of choriocarcinoma-like HCG isoforms secreted by the early trophoblast is predominant and may be the result of an early developmental regulation of glycosylation enzyme.</description><identifier>ISSN: 0268-1161</identifier><identifier>ISSN: 1460-2350</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/15.10.2209</identifier><identifier>PMID: 11006201</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amenorrhea - blood ; Animals ; bioactivity ; Biological and medical sciences ; Biological Assay - methods ; choriocarcinoma ; Chorionic Gonadotropin - blood ; Chorionic Gonadotropin - immunology ; Chorionic Gonadotropin - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; HCG ; Humans ; Leydig Cells - immunology ; Leydig Cells - metabolism ; Male ; Mice ; monoclonal antibodies ; Mother. Fetoplacental unit. Mammary gland. Milk ; Pregnancy ; Pregnancy Trimester, First - blood ; Pregnancy, Ectopic - blood ; Pregnancy. Parturition. Lactation ; Protein Isoforms - blood ; Protein Isoforms - metabolism ; Retrospective Studies ; Vertebrates: reproduction</subject><ispartof>Human reproduction (Oxford), 2000-10, Vol.15 (10), p.2209-2214</ispartof><rights>European Society of Human Reproduction and Embryology 2000</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-a9c0341b40cfbcb40e6dde7e3bf716863661e34e09d4a810091c2a48e57d66703</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1509267$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11006201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mock, Pascal</creatorcontrib><creatorcontrib>Kovalevskaya, Galina</creatorcontrib><creatorcontrib>O'Connor, John F.</creatorcontrib><creatorcontrib>Campana, Aldo</creatorcontrib><title>Choriocarcinoma-like human chorionic gonadotrophin (HCG) and HCG bioactivity during the first trimester of pregnancy</title><title>Human reproduction (Oxford)</title><addtitle>Hum. Reprod</addtitle><addtitle>Hum. Reprod</addtitle><description>The objective of this study was to evaluate the distribution of choriocarcinoma-like human chorionic gonadotrophin (HCG) isoforms during first trimester pregnancy and their relationship with in-vitro HCG bioactivity. This was done by means of a retrospective analysis of patients' sera with first trimester normal intrauterine and abnormal (ectopic) pregnancies. Serum samples were obtained from 38 women with an amenorrhoea of <10 weeks. From these, 19 had a normal intrauterine pregnancy (IUP) and 19 an ectopic pregnancy (EP). Total immunoreactive HCG (HCGi), free β-HCGi and oestradiol were measured by enzyme immunoassays and bioactive HCG by the mouse Leydig cell bioassay. The alterations in HCG isoform content were measured by the combination of two immunometric assays, B152 for choriocarcinoma-like HCG and B109 for intact HCG detection and expressed as the B152/B109 ratio. Choriocarcinoma-like HCG isoforms ratio measured by B152 and B109 assays was significantly higher in the low subgroups of free β-HCGi and gestational age (P = 0.0111 and 0.0036 respectively). Whereas bioactive to immunoreactive HCG ratios (b/i ratio) were significantly higher when free β-HCGi concentrations were low (P = 0.0010), no correlation was found between the variation of bioactivity (b/i ratio) and the proportion of choriocarcinoma-like HCG isoforms (B159/B108). It is concluded that in first trimester pregnancies (i) the modulation of HCG in-vitro bioactivity is not related to the variation of choriocarcinoma-like HCG isoforms secretion and (ii) the amount of choriocarcinoma-like HCG isoforms secreted by the early trophoblast is predominant and may be the result of an early developmental regulation of glycosylation enzyme.</description><subject>Amenorrhea - blood</subject><subject>Animals</subject><subject>bioactivity</subject><subject>Biological and medical sciences</subject><subject>Biological Assay - methods</subject><subject>choriocarcinoma</subject><subject>Chorionic Gonadotropin - blood</subject><subject>Chorionic Gonadotropin - immunology</subject><subject>Chorionic Gonadotropin - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HCG</subject><subject>Humans</subject><subject>Leydig Cells - immunology</subject><subject>Leydig Cells - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>monoclonal antibodies</subject><subject>Mother. Fetoplacental unit. Mammary gland. Milk</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, First - blood</subject><subject>Pregnancy, Ectopic - blood</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Protein Isoforms - blood</subject><subject>Protein Isoforms - metabolism</subject><subject>Retrospective Studies</subject><subject>Vertebrates: reproduction</subject><issn>0268-1161</issn><issn>1460-2350</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFGP1CAUhYnRuOPqD_DF8GCMJnb3Qiktj6bRHZNN1I0a4wuhlM7gtlCBGuffy9iJ--rTvcB3DvcehJ4SuCAgysv9MgUzX5IqHy8oBXEPbQjjUNCygvtoA5Q3BSGcnKFHMf4AyG3DH6IzQgA4BbJBqd37YL1WQVvnJ1WM9tbgbKwc1n-fnNV4553qfQp-3luHX27bq1dYuR7nBnfWK53sL5sOuF-CdTuc9gYPNsSEU7CTickE7Ac8B7NzyunDY_RgUGM0T071HH159_Zzuy2uP1y9b99cF5oxlgolNJSMdAz00OlcDO97U5uyG2rCG15yTkzJDIieqSavJIimijWmqnvOayjP0YvVdw7-55LnkJON2oyjcsYvUdaUCsEFzyBZQR18jMEMcs6Dq3CQBOQxarlGLUl1vDlGnTXPTuZLN5n-TnHKNgPPT4CKWo1DyKvbeMdVICivM_Z6xfwy_9e3xYrbHOvvfwIVbmU2qyu5_fZd3tCvn262H0G25R-V0KbY</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Mock, Pascal</creator><creator>Kovalevskaya, Galina</creator><creator>O'Connor, John F.</creator><creator>Campana, Aldo</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001001</creationdate><title>Choriocarcinoma-like human chorionic gonadotrophin (HCG) and HCG bioactivity during the first trimester of pregnancy</title><author>Mock, Pascal ; Kovalevskaya, Galina ; O'Connor, John F. ; Campana, Aldo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-a9c0341b40cfbcb40e6dde7e3bf716863661e34e09d4a810091c2a48e57d66703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amenorrhea - blood</topic><topic>Animals</topic><topic>bioactivity</topic><topic>Biological and medical sciences</topic><topic>Biological Assay - methods</topic><topic>choriocarcinoma</topic><topic>Chorionic Gonadotropin - blood</topic><topic>Chorionic Gonadotropin - immunology</topic><topic>Chorionic Gonadotropin - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HCG</topic><topic>Humans</topic><topic>Leydig Cells - immunology</topic><topic>Leydig Cells - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>monoclonal antibodies</topic><topic>Mother. Fetoplacental unit. Mammary gland. Milk</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, First - blood</topic><topic>Pregnancy, Ectopic - blood</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Protein Isoforms - blood</topic><topic>Protein Isoforms - metabolism</topic><topic>Retrospective Studies</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mock, Pascal</creatorcontrib><creatorcontrib>Kovalevskaya, Galina</creatorcontrib><creatorcontrib>O'Connor, John F.</creatorcontrib><creatorcontrib>Campana, Aldo</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mock, Pascal</au><au>Kovalevskaya, Galina</au><au>O'Connor, John F.</au><au>Campana, Aldo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Choriocarcinoma-like human chorionic gonadotrophin (HCG) and HCG bioactivity during the first trimester of pregnancy</atitle><jtitle>Human reproduction (Oxford)</jtitle><stitle>Hum. Reprod</stitle><addtitle>Hum. Reprod</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>15</volume><issue>10</issue><spage>2209</spage><epage>2214</epage><pages>2209-2214</pages><issn>0268-1161</issn><issn>1460-2350</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>The objective of this study was to evaluate the distribution of choriocarcinoma-like human chorionic gonadotrophin (HCG) isoforms during first trimester pregnancy and their relationship with in-vitro HCG bioactivity. This was done by means of a retrospective analysis of patients' sera with first trimester normal intrauterine and abnormal (ectopic) pregnancies. Serum samples were obtained from 38 women with an amenorrhoea of <10 weeks. From these, 19 had a normal intrauterine pregnancy (IUP) and 19 an ectopic pregnancy (EP). Total immunoreactive HCG (HCGi), free β-HCGi and oestradiol were measured by enzyme immunoassays and bioactive HCG by the mouse Leydig cell bioassay. The alterations in HCG isoform content were measured by the combination of two immunometric assays, B152 for choriocarcinoma-like HCG and B109 for intact HCG detection and expressed as the B152/B109 ratio. Choriocarcinoma-like HCG isoforms ratio measured by B152 and B109 assays was significantly higher in the low subgroups of free β-HCGi and gestational age (P = 0.0111 and 0.0036 respectively). Whereas bioactive to immunoreactive HCG ratios (b/i ratio) were significantly higher when free β-HCGi concentrations were low (P = 0.0010), no correlation was found between the variation of bioactivity (b/i ratio) and the proportion of choriocarcinoma-like HCG isoforms (B159/B108). It is concluded that in first trimester pregnancies (i) the modulation of HCG in-vitro bioactivity is not related to the variation of choriocarcinoma-like HCG isoforms secretion and (ii) the amount of choriocarcinoma-like HCG isoforms secreted by the early trophoblast is predominant and may be the result of an early developmental regulation of glycosylation enzyme.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>11006201</pmid><doi>10.1093/humrep/15.10.2209</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Amenorrhea - blood Animals bioactivity Biological and medical sciences Biological Assay - methods choriocarcinoma Chorionic Gonadotropin - blood Chorionic Gonadotropin - immunology Chorionic Gonadotropin - metabolism Female Fundamental and applied biological sciences. Psychology HCG Humans Leydig Cells - immunology Leydig Cells - metabolism Male Mice monoclonal antibodies Mother. Fetoplacental unit. Mammary gland. Milk Pregnancy Pregnancy Trimester, First - blood Pregnancy, Ectopic - blood Pregnancy. Parturition. Lactation Protein Isoforms - blood Protein Isoforms - metabolism Retrospective Studies Vertebrates: reproduction |
| title | Choriocarcinoma-like human chorionic gonadotrophin (HCG) and HCG bioactivity during the first trimester of pregnancy |
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