Effects of Vasodilatory Antihypertensive Agents on Endothelial Dysfunction in Rats with Ischemic Acute Renal Failure

Ischemic acute renal failure is associated with vascular endothelial dysfunction. We examined whether vasodilatory antihypertensive agents would improve endothelial function in rats with ischemia/reperfusion renal injury. Rat kidneys were isolated and perfused after clipping of the bilateral renal arteries for 45min and reperfusion for 24h, and renal perfusion pressure and nitric oxide concentration in the venous effluent (chemiluminescence assay) were monitored. Preischemic administration of celiprolol (a β-blocker; 100mg/kg p.o.), benidipine (a calcium channel blocker; 1mg/kg p.o.), or imidapril (an angiotensin converting-enzyme inhibitor; 3mg/kg p.o.) restored endothelial function in rats subjected to acute renal ischemia (Δrenal perfusion pressure [10-8M acetylcholine]: sham -42±3%, ischemia -31±1%, ischemia +celiprolol -39±1%*, ischemia+benidipine -38±2%*, ischemia+imidapril -42±2%*; *p