Biosurfactants of MEL-A Increase Gene Transfection Mediated by Cationic Liposomes

Many microorganisms growing on water-insoluble substrates have been known to produce surface-active compounds called biosurfactants. Although biosurfactants have received increasing attention due to their special properties, there has been no information available until now of a role for them with r...

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Veröffentlicht in:Biochemical and biophysical research communications 2001-11, Vol.289 (1), p.57-61
Hauptverfasser: Inoh, Yoshikazu, Kitamoto, Dai, Hirashima, Naohide, Nakanishi, Mamoru
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Sprache:eng
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Zusammenfassung:Many microorganisms growing on water-insoluble substrates have been known to produce surface-active compounds called biosurfactants. Although biosurfactants have received increasing attention due to their special properties, there has been no information available until now of a role for them with regard to gene transfection. Thus, we studied here the effects of biosurfactants on gene transfection by cationic liposomes with a cationic cholesterol derivative. Our results showed clearly that a biosurfactant of mannosylerythritol lipid A (MEL-A) increased dramatically the efficiency of gene transfection mediated by cationic liposomes with a cationic cholesterol derivative. Among them, the liposomes with a cationic cholesterol derivative, cholesteryl-3β-carboxyamindoethylene-N-hydroxyethylamine (I), were much more effective for gene transfection than the liposomes with DC-Chol (cholesteryl-3β-oxycarboxyamidoethylenedimethylamine) or liposomes without MEL-A in various cultured cells. This demonstrates that this new finding has great potential in the experiment of gene transfection and gene therapy mediated by nonviral vectors such as cationic liposomes.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5930