Separation and Quantitation of Bile Acids Using an Isocratic Solvent System for High Performance Liquid Chromatography Coupled to an Evaporative Light Scattering Detector
We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rod...
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| Veröffentlicht in: | Analytical biochemistry 2001-11, Vol.298 (2), p.293-298 |
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| container_end_page | 298 |
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| container_title | Analytical biochemistry |
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| creator | Torchia, Enrique C. Labonté, Eric D. Agellon, Luis B. |
| description | We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids. |
| doi_str_mv | 10.1006/abio.2001.5379 |
| format | Article |
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An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1006/abio.2001.5379</identifier><identifier>PMID: 11700985</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bile - chemistry ; bile acids ; Bile Acids and Salts - analysis ; Bile Acids and Salts - isolation & purification ; Chromatography, High Pressure Liquid - methods ; evaporative light scattering detector ; Gallbladder - chemistry ; high performance liquid chromatography ; Humans ; Light ; Scattering, Radiation ; Solvents</subject><ispartof>Analytical biochemistry, 2001-11, Vol.298 (2), p.293-298</ispartof><rights>2001 Academic Press</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-6a1de97745f03cee6ca6fc1012286c612ea83518841be00eb627e9c25c1262ed3</citedby><cites>FETCH-LOGICAL-c406t-6a1de97745f03cee6ca6fc1012286c612ea83518841be00eb627e9c25c1262ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003269701953795$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11700985$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torchia, Enrique C.</creatorcontrib><creatorcontrib>Labonté, Eric D.</creatorcontrib><creatorcontrib>Agellon, Luis B.</creatorcontrib><title>Separation and Quantitation of Bile Acids Using an Isocratic Solvent System for High Performance Liquid Chromatography Coupled to an Evaporative Light Scattering Detector</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids.</description><subject>Animals</subject><subject>Bile - chemistry</subject><subject>bile acids</subject><subject>Bile Acids and Salts - analysis</subject><subject>Bile Acids and Salts - isolation & purification</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>evaporative light scattering detector</subject><subject>Gallbladder - chemistry</subject><subject>high performance liquid chromatography</subject><subject>Humans</subject><subject>Light</subject><subject>Scattering, Radiation</subject><subject>Solvents</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kbFu2zAQhomiReKkWTsWnLrJPVIWJY2pmyYBDLSFm5mgyZPNQhIVkjLgV-pTloQNdOrEA_Hdd7j7CfnAYMkAxGe1s27JAdiyKuv2DVkwaEUBJbRvyQIAyoKLtr4mNyH8ThRbVeKKXDNWA7RNtSB_tjgpr6J1I1WjoT9nNUYbzx-uo19sj_ReWxPoS7DjPkH0OTidWzTduv6IY6TbU4g40M55-mT3B_oDfaoHNWqkG_s6W0PXB-8GFd3eq-lwoms3Tz0aGl02PhzV5LLymPn9IRm1ihF9nvgVI-ro_HvyrlN9wLvLe0tevj38Wj8Vm--Pz-v7TaFXIGIhFDPY1vWq6qDUiEIr0WkGjPNGaME4qqasWNOs2A4BcCd4ja3mlWZccDTlLfl09k7evc4Yohxs0Nj3akQ3B1lzLipWiQQuz6D2LgSPnZy8HZQ_SQYypyNzOjKnI3M6qeHjxTzvBjT_8EscCWjOAKb9jha9DNpiuqKxPt1AGmf_5_4Lq_ahYQ</recordid><startdate>20011115</startdate><enddate>20011115</enddate><creator>Torchia, Enrique C.</creator><creator>Labonté, Eric D.</creator><creator>Agellon, Luis B.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011115</creationdate><title>Separation and Quantitation of Bile Acids Using an Isocratic Solvent System for High Performance Liquid Chromatography Coupled to an Evaporative Light Scattering Detector</title><author>Torchia, Enrique C. ; Labonté, Eric D. ; Agellon, Luis B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-6a1de97745f03cee6ca6fc1012286c612ea83518841be00eb627e9c25c1262ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Bile - chemistry</topic><topic>bile acids</topic><topic>Bile Acids and Salts - analysis</topic><topic>Bile Acids and Salts - isolation & purification</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>evaporative light scattering detector</topic><topic>Gallbladder - chemistry</topic><topic>high performance liquid chromatography</topic><topic>Humans</topic><topic>Light</topic><topic>Scattering, Radiation</topic><topic>Solvents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Torchia, Enrique C.</creatorcontrib><creatorcontrib>Labonté, Eric D.</creatorcontrib><creatorcontrib>Agellon, Luis B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torchia, Enrique C.</au><au>Labonté, Eric D.</au><au>Agellon, Luis B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation and Quantitation of Bile Acids Using an Isocratic Solvent System for High Performance Liquid Chromatography Coupled to an Evaporative Light Scattering Detector</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2001-11-15</date><risdate>2001</risdate><volume>298</volume><issue>2</issue><spage>293</spage><epage>298</epage><pages>293-298</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11700985</pmid><doi>10.1006/abio.2001.5379</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 2001-11, Vol.298 (2), p.293-298 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72265156 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bile - chemistry bile acids Bile Acids and Salts - analysis Bile Acids and Salts - isolation & purification Chromatography, High Pressure Liquid - methods evaporative light scattering detector Gallbladder - chemistry high performance liquid chromatography Humans Light Scattering, Radiation Solvents |
| title | Separation and Quantitation of Bile Acids Using an Isocratic Solvent System for High Performance Liquid Chromatography Coupled to an Evaporative Light Scattering Detector |
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