Separation and Quantitation of Bile Acids Using an Isocratic Solvent System for High Performance Liquid Chromatography Coupled to an Evaporative Light Scattering Detector

We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rod...

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Veröffentlicht in:	Analytical biochemistry 2001-11, Vol.298 (2), p.293-298
Hauptverfasser:	Torchia, Enrique C., Labonté, Eric D., Agellon, Luis B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bile - chemistry bile acids Bile Acids and Salts - analysis Bile Acids and Salts - isolation & purification Chromatography, High Pressure Liquid - methods evaporative light scattering detector Gallbladder - chemistry high performance liquid chromatography Humans Light Scattering, Radiation Solvents
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Beschreibung
Zusammenfassung:	We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids.
ISSN:	0003-2697 1096-0309
DOI:	10.1006/abio.2001.5379