Miniaturization of capillary isoelectric focusing
Miniaturization of whole‐column imaging capillary isoelectric focusing (CIEF) is discussed. A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two pI markers and the protein myoglobin showed that good CIEF separation results could be obtained. S...
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| Veröffentlicht in: | Electrophoresis 2001-10, Vol.22 (18), p.3968-3971 |
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| container_title | Electrophoresis |
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| creator | Wu, Xing-Zheng Sze, Newman S.-K. Pawliszyn, Janusz |
| description | Miniaturization of whole‐column imaging capillary isoelectric focusing (CIEF) is discussed. A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two pI markers and the protein myoglobin showed that good CIEF separation results could be obtained. Secondly, a light‐emitting diode (LED) was used as the light source for the whole‐column absorbance imaging detection. The focusing of both the pI markers and myoglobin were observed with the LED light source. The whole‐column imaging CIEF instrument was simplified and miniaturized by the use of the LED. Further developments are also discussed. |
| doi_str_mv | 10.1002/1522-2683(200110)22:18<3968::AID-ELPS3968>3.0.CO;2-9 |
| format | Article |
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A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two pI markers and the protein myoglobin showed that good CIEF separation results could be obtained. Secondly, a light‐emitting diode (LED) was used as the light source for the whole‐column absorbance imaging detection. The focusing of both the pI markers and myoglobin were observed with the LED light source. The whole‐column imaging CIEF instrument was simplified and miniaturized by the use of the LED. Further developments are also discussed.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/1522-2683(200110)22:18<3968::AID-ELPS3968>3.0.CO;2-9</identifier><identifier>PMID: 11700727</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Capillary isoelectric focusing ; Electrophoresis, Capillary - instrumentation ; Equipment Design ; Isoelectric Focusing - instrumentation ; Isoelectric Point ; Light ; Microchemistry - instrumentation ; Miniaturization ; Myoglobin - chemistry ; Protein Isoforms - chemistry ; Whole-column imaging</subject><ispartof>Electrophoresis, 2001-10, Vol.22 (18), p.3968-3971</ispartof><rights>Copyright © 2001 WILEY‐VCH Verlag GmbH, Weinheim, Fed. 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A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two pI markers and the protein myoglobin showed that good CIEF separation results could be obtained. Secondly, a light‐emitting diode (LED) was used as the light source for the whole‐column absorbance imaging detection. The focusing of both the pI markers and myoglobin were observed with the LED light source. The whole‐column imaging CIEF instrument was simplified and miniaturized by the use of the LED. Further developments are also discussed.</description><subject>Animals</subject><subject>Capillary isoelectric focusing</subject><subject>Electrophoresis, Capillary - instrumentation</subject><subject>Equipment Design</subject><subject>Isoelectric Focusing - instrumentation</subject><subject>Isoelectric Point</subject><subject>Light</subject><subject>Microchemistry - instrumentation</subject><subject>Miniaturization</subject><subject>Myoglobin - chemistry</subject><subject>Protein Isoforms - chemistry</subject><subject>Whole-column imaging</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE1vEzEQhi0EakPpX0A5VfSwYWzv-iNUSFVa0pZAiqiEeho5zhgZNtl0vSsov55dJYQTB07WWDPPO_MwdsZhxAHEa14IkQll5CsBwDmcCjHm5kxaZcbj8-uL7HJ2-7mv3soRjCbzNyKzT9hgP_aUDYBrmYGRxSF7ntI3AMhtnh-wQ841gBZ6wPiHuI6uaev4yzWxWg-rMPRuE8vS1Y_DmCoqyTd19MNQ-TbF9dcX7FlwZaLj3XvE7t5d3k2ustl8ej05n2U-12AyGSi4JdhCqyUnIg5EprCF4rkiYTVJ7wsDyngPPoDIrTNO2yBosVAqyCN2ssVu6uqhpdTgKiZP3V5rqtqEWgjVXaHkPt_XVUo1BdzUcdVtjxywN4m9EuyV4NYkdiU32LtD7EziH5MoEXAyR4G2w77c5beLFS3_Qnfquob7bcOPWNLjf4X-I3P_17GzLTumhn7u2a7-jkpLXeCXj1MsPvHbi_c3CqfyN7YenJo</recordid><startdate>200110</startdate><enddate>200110</enddate><creator>Wu, Xing-Zheng</creator><creator>Sze, Newman S.-K.</creator><creator>Pawliszyn, Janusz</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200110</creationdate><title>Miniaturization of capillary isoelectric focusing</title><author>Wu, Xing-Zheng ; Sze, Newman S.-K. ; Pawliszyn, Janusz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4708-3fefad09576d1eee10ee85956146e297e3cc58068cc0cf0249a8a79f2ebb66f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Capillary isoelectric focusing</topic><topic>Electrophoresis, Capillary - instrumentation</topic><topic>Equipment Design</topic><topic>Isoelectric Focusing - instrumentation</topic><topic>Isoelectric Point</topic><topic>Light</topic><topic>Microchemistry - instrumentation</topic><topic>Miniaturization</topic><topic>Myoglobin - chemistry</topic><topic>Protein Isoforms - chemistry</topic><topic>Whole-column imaging</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Xing-Zheng</creatorcontrib><creatorcontrib>Sze, Newman S.-K.</creatorcontrib><creatorcontrib>Pawliszyn, Janusz</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Xing-Zheng</au><au>Sze, Newman S.-K.</au><au>Pawliszyn, Janusz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Miniaturization of capillary isoelectric focusing</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>2001-10</date><risdate>2001</risdate><volume>22</volume><issue>18</issue><spage>3968</spage><epage>3971</epage><pages>3968-3971</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Miniaturization of whole‐column imaging capillary isoelectric focusing (CIEF) is discussed. A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two pI markers and the protein myoglobin showed that good CIEF separation results could be obtained. Secondly, a light‐emitting diode (LED) was used as the light source for the whole‐column absorbance imaging detection. The focusing of both the pI markers and myoglobin were observed with the LED light source. The whole‐column imaging CIEF instrument was simplified and miniaturized by the use of the LED. Further developments are also discussed.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>11700727</pmid><doi>10.1002/1522-2683(200110)22:18<3968::AID-ELPS3968>3.0.CO;2-9</doi><tpages>4</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Capillary isoelectric focusing Electrophoresis, Capillary - instrumentation Equipment Design Isoelectric Focusing - instrumentation Isoelectric Point Light Microchemistry - instrumentation Miniaturization Myoglobin - chemistry Protein Isoforms - chemistry Whole-column imaging |
| title | Miniaturization of capillary isoelectric focusing |
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