Miniaturization of capillary isoelectric focusing

Miniaturization of capillary isoelectric focusing

Miniaturization of whole‐column imaging capillary isoelectric focusing (CIEF) is discussed. A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two pI markers and the protein myoglobin showed that good CIEF separation results could be obtained. S...

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Veröffentlicht in:Electrophoresis 2001-10, Vol.22 (18), p.3968-3971
Hauptverfasser: Wu, Xing-Zheng, Sze, Newman S.-K., Pawliszyn, Janusz
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Capillary isoelectric focusing
Electrophoresis, Capillary - instrumentation
Equipment Design
Isoelectric Focusing - instrumentation
Isoelectric Point
Light
Microchemistry - instrumentation
Miniaturization
Myoglobin - chemistry
Protein Isoforms - chemistry
Whole-column imaging
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Zusammenfassung:Miniaturization of whole‐column imaging capillary isoelectric focusing (CIEF) is discussed. A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two pI markers and the protein myoglobin showed that good CIEF separation results could be obtained. Secondly, a light‐emitting diode (LED) was used as the light source for the whole‐column absorbance imaging detection. The focusing of both the pI markers and myoglobin were observed with the LED light source. The whole‐column imaging CIEF instrument was simplified and miniaturized by the use of the LED. Further developments are also discussed.
ISSN:0173-0835
1522-2683
DOI:10.1002/1522-2683(200110)22:18<3968::AID-ELPS3968>3.0.CO;2-9