Thyroid hormone coordinates respiratory control maturation and adenine nucleotide translocator expression in heart in vivo

The signal transduction mechanism linking mitochondrial ATP synthesis with cytosolic ATP utilization in heart changes during postnatal development in vivo. This maturational process occurs in parallel with accumulation of mitochondrial adenine nucleotide translocator (ANT), which provides a possible...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Circulation (New York, N.Y.) N.Y.), 2000-09, Vol.102 (11), p.1323-1329
Hauptverfasser:	PORTMAN, Michael A, YUN XIAO, KUN QIAN, TUCKER, Russell L, PARISH, Steven M, NING, Xue-Han
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate - metabolism Animals Animals, Newborn - physiology Biological and medical sciences Blotting, Northern Blotting, Western Cardiovascular system Hemodynamics Hydrogen-Ion Concentration Intracellular Fluid - enzymology Intracellular Fluid - metabolism Investigative techniques, diagnostic techniques (general aspects) Isoenzymes - metabolism Magnetic Resonance Spectroscopy Medical sciences Mitochondrial ADP, ATP Translocases - metabolism Myocardium - enzymology Myocardium - metabolism Oxygen Consumption Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Phosphocreatine - metabolism Proton-Translocating ATPases - metabolism Respiratory Physiological Phenomena RNA, Messenger - metabolism Sheep Thyroidectomy Thyroxine - blood Thyroxine - physiology Triiodothyronine - blood Triiodothyronine - physiology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The signal transduction mechanism linking mitochondrial ATP synthesis with cytosolic ATP utilization in heart changes during postnatal development in vivo. This maturational process occurs in parallel with accumulation of mitochondrial adenine nucleotide translocator (ANT), which provides a possible site for respiratory control. We postulated that thyroid hormone regulates these maturational processes. We used (31)P MR spectroscopy to determine the relationship between myocardial high-energy phosphates, phosphocreatine, and ADP and oxygen consumption (MVO(2)) during epinephrine stimulation in 32- to 40-day-old lambs thyroidectomized after birth (THY) and age-matched controls. Steady-state protein and mRNA levels for ANT isoforms and beta-F(1)-ATPase were assessed from left ventricular tissues by Western and Northern blotting. With greater doses of epinephrine, THY attained lower peak MVO(2) than controls (P:
ISSN:	0009-7322 1524-4539
DOI:	10.1161/01.CIR.102.11.1323