Inhibition of Purified Recombinant Reverse Transcriptase from Wild-Type and Zidovudine-Resistant Clinical Isolates of Human Immunodeficiency Virus Type 1 by Zidovudine, Stavudine, and Lamivudine Triphosphates

Cross-resistance between zidovudine, stavudine, and lamivudine was studied, using purified recombinant reverse transcriptase from a zidovudine-susceptible and -resistant pair of clinical isolates of human immunodeficiency virus type 1. The zidovudine-resistant isolate exhibited low-level cross-resis...

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Veröffentlicht in:The Journal of infectious diseases 2001-11, Vol.184 (10), p.1336-1340
Hauptverfasser: Duan, Chengying, Poticha, David, Stoeckli, Thomas C., Petropoulos, Christos J., Whitcomb, Jeannette M., McHenry, Charles S., Kuritzkes, Daniel R.
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Sprache:eng
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Zusammenfassung:Cross-resistance between zidovudine, stavudine, and lamivudine was studied, using purified recombinant reverse transcriptase from a zidovudine-susceptible and -resistant pair of clinical isolates of human immunodeficiency virus type 1. The zidovudine-resistant isolate exhibited low-level cross-resistance to both stavudine and lamivudine in drug susceptibility assays. Enzyme from the resistant isolate demonstrated reduced inhibition by zidovudine triphosphate and stavudine triphosphate and, to a lesser extent, lamivudine triphosphate. These findings provide additional evidence at the viral and enzyme level for cross-resistance between zidovudine and stavudine, and they suggest a possible effect of zidovudine resistance on susceptibility to lamivudine.
ISSN:0022-1899
1537-6613
DOI:10.1086/323995