Allergen-specific T cell reactivity in cord blood: the influence of maternal cytokine production

Background Successful pregnancy is dependent upon T helper (Th)2‐type‐dominated immunological responsiveness in gestation‐associated compartments. Objective In our study we observed the influence of the maternal Th2‐associated cytokine pattern on the naive fetal T cell phenotype and asked if circula...

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Veröffentlicht in:Clinical and experimental allergy 2001-10, Vol.31 (10), p.1536-1543
Hauptverfasser: Kopp, M. V., Zehle, C., Pichler, J., Szépfalusi, Z., Moseler, M., Deichmann, K., Forster, J., Kuehr, J.
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Sprache:eng
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Zusammenfassung:Background Successful pregnancy is dependent upon T helper (Th)2‐type‐dominated immunological responsiveness in gestation‐associated compartments. Objective In our study we observed the influence of the maternal Th2‐associated cytokine pattern on the naive fetal T cell phenotype and asked if circulating Th2 cytokines of atopic mothers affects the Th1/Th2 differentiation of the fetus. Methods Cord blood mononuclear cells (CBMC) and peripheral blood mononuclear cells (PBMC) of the corresponding mothers were isolated. The proliferative response of CBMC and PBMC to Betalactoglobulin (BLG) was assessed by liquid scintillation counting. The cytokines interferon (IFN)‐γ, and interleukin (IL)‐5, IL‐10 and IL‐13 in the cell culture supernatants were measured using the ELISA technique. We then defined two subgroups based on maternal levels of specific IgE against aeroallergens: sensitized mothers (MA+) and their neonates (NMA+) (n = 18) and non‐sensitized mothers (MA−) and their neonates (NMA−) (n = 29). Results Nearly all mothers (98%) and neonates (92%) had a positive proliferation response after stimulation with BLG (mean stimulation index (10–90 percentile): neonates: 7 (2–15); mothers 14 (5–29)). In supernatants of BLG‐stimulated cell cultures, sensitized mothers showed a significantly lower IFN‐γ concentration in comparison to non‐sensitized mothers (MA+ = 25; MA− = 123 IU/L; P 
ISSN:0954-7894
1365-2222
DOI:10.1046/j.1365-2222.2001.01198.x