Dietary lipids affect human ethanol-inducible CYP2E1 gene expression in vivo in mononuclear cells
We tested the hypothesis that dietary cholesterol modulate human ethanol-inducible CYP2E1 expression in vivo in circulating mononuclear cells. Healthy volunteers (n=10) were submitted to a low fat low cholesterol diet for 4 days (day 0–day 3, LFLC). Cholesterol (595±56 mg/day) was then re-introduced...
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| Veröffentlicht in: | Life sciences (1973) 2000-08, Vol.67 (11), p.1307-1316 |
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| container_title | Life sciences (1973) |
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| creator | Boucher, Philippe Seree, Eric Vidon, Caroline de Souza, Anna Cachefo Villard, Pierre Henri Chambon, Renée Barra, Yves Vallon, Jean Jacques |
| description | We tested the hypothesis that dietary cholesterol modulate human ethanol-inducible CYP2E1 expression
in vivo in circulating mononuclear cells. Healthy volunteers (n=10) were submitted to a low fat low cholesterol diet for 4 days (day 0–day 3, LFLC). Cholesterol (595±56 mg/day) was then re-introduced for 7 days (day 4–day 10, LFHC). In the same time, controls subjects (n=7) did not change their habitual daily diet. CYP2E1 mRNA levels, evaluated in mononuclear cells, decreased in experimental subjects during both LFLC and LFHC from 100% to 53±5%, (p |
| doi_str_mv | 10.1016/S0024-3205(00)00721-9 |
| format | Article |
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in vivo in circulating mononuclear cells. Healthy volunteers (n=10) were submitted to a low fat low cholesterol diet for 4 days (day 0–day 3, LFLC). Cholesterol (595±56 mg/day) was then re-introduced for 7 days (day 4–day 10, LFHC). In the same time, controls subjects (n=7) did not change their habitual daily diet. CYP2E1 mRNA levels, evaluated in mononuclear cells, decreased in experimental subjects during both LFLC and LFHC from 100% to 53±5%, (p<0.001) with a main decrease during LFLC period (100% to 71±16%, p=0.05). Immunoreactive CYP2E1 showed a similar pattern and decreased from 100 to 62±12% during the trial (p<0.05). No significant change occured in control subjects. Between day 0 and day 11, changes in CYP2E1 mRNA correlated positively with plasma cholesterol (r
2=0.67, p<0.001) and HDL cholesterol concentrations (r
2=0.61, p<0.001). In contrast, no correlation was found between plasma fatty acids concentrations and CYP2E1 expression. The present results suggest that lipid factors regulate CYP2E1 expression, in vivo, in human mononuclear cells. In particular, plasma cholesterol concentrations may play an important role in this regulation.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/S0024-3205(00)00721-9</identifier><identifier>PMID: 10972199</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Adult ; Base Sequence ; Blood Glucose - analysis ; Cholesterol ; CYP2E1 ; Cytochrome P-450 CYP2E1 - genetics ; Cytochrome P-450 CYP2E1 - metabolism ; Dietary Fats - pharmacology ; DNA Primers ; Ethanol - pharmacology ; Ethanol toxicity ; Fatty acids ; Female ; Gene Expression Regulation, Enzymologic - drug effects ; Humans ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - enzymology ; Lipids - blood ; Male ; Middle Aged ; Mononuclear cells ; RNA, Messenger - genetics ; RNA, Messenger - metabolism</subject><ispartof>Life sciences (1973), 2000-08, Vol.67 (11), p.1307-1316</ispartof><rights>2000 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-679cdb671ea4cb7af4d6effdc93c29986eaed00862313e369d6f0cb3627b7d043</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0024-3205(00)00721-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10972199$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boucher, Philippe</creatorcontrib><creatorcontrib>Seree, Eric</creatorcontrib><creatorcontrib>Vidon, Caroline</creatorcontrib><creatorcontrib>de Souza, Anna Cachefo</creatorcontrib><creatorcontrib>Villard, Pierre Henri</creatorcontrib><creatorcontrib>Chambon, Renée</creatorcontrib><creatorcontrib>Barra, Yves</creatorcontrib><creatorcontrib>Vallon, Jean Jacques</creatorcontrib><title>Dietary lipids affect human ethanol-inducible CYP2E1 gene expression in vivo in mononuclear cells</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>We tested the hypothesis that dietary cholesterol modulate human ethanol-inducible CYP2E1 expression
in vivo in circulating mononuclear cells. Healthy volunteers (n=10) were submitted to a low fat low cholesterol diet for 4 days (day 0–day 3, LFLC). Cholesterol (595±56 mg/day) was then re-introduced for 7 days (day 4–day 10, LFHC). In the same time, controls subjects (n=7) did not change their habitual daily diet. CYP2E1 mRNA levels, evaluated in mononuclear cells, decreased in experimental subjects during both LFLC and LFHC from 100% to 53±5%, (p<0.001) with a main decrease during LFLC period (100% to 71±16%, p=0.05). Immunoreactive CYP2E1 showed a similar pattern and decreased from 100 to 62±12% during the trial (p<0.05). No significant change occured in control subjects. Between day 0 and day 11, changes in CYP2E1 mRNA correlated positively with plasma cholesterol (r
2=0.67, p<0.001) and HDL cholesterol concentrations (r
2=0.61, p<0.001). In contrast, no correlation was found between plasma fatty acids concentrations and CYP2E1 expression. The present results suggest that lipid factors regulate CYP2E1 expression, in vivo, in human mononuclear cells. In particular, plasma cholesterol concentrations may play an important role in this regulation.</description><subject>Adult</subject><subject>Base Sequence</subject><subject>Blood Glucose - analysis</subject><subject>Cholesterol</subject><subject>CYP2E1</subject><subject>Cytochrome P-450 CYP2E1 - genetics</subject><subject>Cytochrome P-450 CYP2E1 - metabolism</subject><subject>Dietary Fats - pharmacology</subject><subject>DNA Primers</subject><subject>Ethanol - pharmacology</subject><subject>Ethanol toxicity</subject><subject>Fatty acids</subject><subject>Female</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Leukocytes, Mononuclear - enzymology</subject><subject>Lipids - blood</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mononuclear cells</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhq0K1G5LH6HIJ0QPacd2Yq9PCC0FKlWiEnDoyXLsSWuU2IudrNq3J-lWiBun__LN_DMfIWcMLhgwefkdgNeV4NC8BzgHUJxV-oCs2FrpCqRgr8jqL3JEjkv5BQBNo8QhOWKgZ17rFbGfAo42P9E-bIMv1HYdupE-TIONFMcHG1NfhegnF9oe6ebull8xeo8RKT5uM5YSUqQh0l3YpSWHFFOcXI82U4d9X96Q153tC56-5An5-fnqx-ZrdfPty_Xm403laq7GSirtfCsVQ1u7Vtmu9hK7zjstHNd6LdGiB1hLLphAIbWXHbhWSK5a5aEWJ-Tdfu82p98TltEMoSwX2IhpKkZxznWz1jPY7EGXUykZO7PNYZgdGAZmcWue3ZpFnAEwz27NMvf2pWBqB_T_TO1lzsCHPYDzm7uA2RQXMDr0Ic9SjU_hPxV_APQliaY</recordid><startdate>20000804</startdate><enddate>20000804</enddate><creator>Boucher, Philippe</creator><creator>Seree, Eric</creator><creator>Vidon, Caroline</creator><creator>de Souza, Anna Cachefo</creator><creator>Villard, Pierre Henri</creator><creator>Chambon, Renée</creator><creator>Barra, Yves</creator><creator>Vallon, Jean Jacques</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000804</creationdate><title>Dietary lipids affect human ethanol-inducible CYP2E1 gene expression in vivo in mononuclear cells</title><author>Boucher, Philippe ; Seree, Eric ; Vidon, Caroline ; de Souza, Anna Cachefo ; Villard, Pierre Henri ; Chambon, Renée ; Barra, Yves ; Vallon, Jean Jacques</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-679cdb671ea4cb7af4d6effdc93c29986eaed00862313e369d6f0cb3627b7d043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Base Sequence</topic><topic>Blood Glucose - analysis</topic><topic>Cholesterol</topic><topic>CYP2E1</topic><topic>Cytochrome P-450 CYP2E1 - genetics</topic><topic>Cytochrome P-450 CYP2E1 - metabolism</topic><topic>Dietary Fats - pharmacology</topic><topic>DNA Primers</topic><topic>Ethanol - pharmacology</topic><topic>Ethanol toxicity</topic><topic>Fatty acids</topic><topic>Female</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Leukocytes, Mononuclear - enzymology</topic><topic>Lipids - blood</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mononuclear cells</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boucher, Philippe</creatorcontrib><creatorcontrib>Seree, Eric</creatorcontrib><creatorcontrib>Vidon, Caroline</creatorcontrib><creatorcontrib>de Souza, Anna Cachefo</creatorcontrib><creatorcontrib>Villard, Pierre Henri</creatorcontrib><creatorcontrib>Chambon, Renée</creatorcontrib><creatorcontrib>Barra, Yves</creatorcontrib><creatorcontrib>Vallon, Jean Jacques</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boucher, Philippe</au><au>Seree, Eric</au><au>Vidon, Caroline</au><au>de Souza, Anna Cachefo</au><au>Villard, Pierre Henri</au><au>Chambon, Renée</au><au>Barra, Yves</au><au>Vallon, Jean Jacques</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dietary lipids affect human ethanol-inducible CYP2E1 gene expression in vivo in mononuclear cells</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2000-08-04</date><risdate>2000</risdate><volume>67</volume><issue>11</issue><spage>1307</spage><epage>1316</epage><pages>1307-1316</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>We tested the hypothesis that dietary cholesterol modulate human ethanol-inducible CYP2E1 expression
in vivo in circulating mononuclear cells. Healthy volunteers (n=10) were submitted to a low fat low cholesterol diet for 4 days (day 0–day 3, LFLC). Cholesterol (595±56 mg/day) was then re-introduced for 7 days (day 4–day 10, LFHC). In the same time, controls subjects (n=7) did not change their habitual daily diet. CYP2E1 mRNA levels, evaluated in mononuclear cells, decreased in experimental subjects during both LFLC and LFHC from 100% to 53±5%, (p<0.001) with a main decrease during LFLC period (100% to 71±16%, p=0.05). Immunoreactive CYP2E1 showed a similar pattern and decreased from 100 to 62±12% during the trial (p<0.05). No significant change occured in control subjects. Between day 0 and day 11, changes in CYP2E1 mRNA correlated positively with plasma cholesterol (r
2=0.67, p<0.001) and HDL cholesterol concentrations (r
2=0.61, p<0.001). In contrast, no correlation was found between plasma fatty acids concentrations and CYP2E1 expression. The present results suggest that lipid factors regulate CYP2E1 expression, in vivo, in human mononuclear cells. In particular, plasma cholesterol concentrations may play an important role in this regulation.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>10972199</pmid><doi>10.1016/S0024-3205(00)00721-9</doi><tpages>10</tpages></addata></record> |
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| subjects | Adult Base Sequence Blood Glucose - analysis Cholesterol CYP2E1 Cytochrome P-450 CYP2E1 - genetics Cytochrome P-450 CYP2E1 - metabolism Dietary Fats - pharmacology DNA Primers Ethanol - pharmacology Ethanol toxicity Fatty acids Female Gene Expression Regulation, Enzymologic - drug effects Humans Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - enzymology Lipids - blood Male Middle Aged Mononuclear cells RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | Dietary lipids affect human ethanol-inducible CYP2E1 gene expression in vivo in mononuclear cells |
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