Dietary lipids affect human ethanol-inducible CYP2E1 gene expression in vivo in mononuclear cells

We tested the hypothesis that dietary cholesterol modulate human ethanol-inducible CYP2E1 expression in vivo in circulating mononuclear cells. Healthy volunteers (n=10) were submitted to a low fat low cholesterol diet for 4 days (day 0–day 3, LFLC). Cholesterol (595±56 mg/day) was then re-introduced...

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Veröffentlicht in:	Life sciences (1973) 2000-08, Vol.67 (11), p.1307-1316
Hauptverfasser:	Boucher, Philippe, Seree, Eric, Vidon, Caroline, de Souza, Anna Cachefo, Villard, Pierre Henri, Chambon, Renée, Barra, Yves, Vallon, Jean Jacques
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Base Sequence Blood Glucose - analysis Cholesterol CYP2E1 Cytochrome P-450 CYP2E1 - genetics Cytochrome P-450 CYP2E1 - metabolism Dietary Fats - pharmacology DNA Primers Ethanol - pharmacology Ethanol toxicity Fatty acids Female Gene Expression Regulation, Enzymologic - drug effects Humans Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - enzymology Lipids - blood Male Middle Aged Mononuclear cells RNA, Messenger - genetics RNA, Messenger - metabolism
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Zusammenfassung:	We tested the hypothesis that dietary cholesterol modulate human ethanol-inducible CYP2E1 expression in vivo in circulating mononuclear cells. Healthy volunteers (n=10) were submitted to a low fat low cholesterol diet for 4 days (day 0–day 3, LFLC). Cholesterol (595±56 mg/day) was then re-introduced for 7 days (day 4–day 10, LFHC). In the same time, controls subjects (n=7) did not change their habitual daily diet. CYP2E1 mRNA levels, evaluated in mononuclear cells, decreased in experimental subjects during both LFLC and LFHC from 100% to 53±5%, (p
ISSN:	0024-3205 1879-0631
DOI:	10.1016/S0024-3205(00)00721-9