Matrix-assisted laser desorption/ionization time-of-flight analysis of Bacillus spores using a 2.94 microm infrared laser

Matrix-assisted laser desorption/ionization time-of-flight analysis of Bacillus spores using a 2.94 microm infrared laser

The performance of infrared (2.94 microm) and ultraviolet (337 nm) lasers were compared for analysis of purified spores of B. subtilis, B. cereus and B. globigii on a four-inch end-cap reflectron time-of-flight instrument. Infrared matrix-assisted laser desorption/ionization (IR-MALDI) mass spectra...

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Veröffentlicht in:Rapid communications in mass spectrometry 2000, Vol.14 (18), p.1701-1706
Hauptverfasser: Ryzhov, V, Bundy, J L, Fenselau, C, Taranenko, N, Doroshenko, V, Prasad, C R
Format: Artikel
Sprache:eng
Schlagworte:
Bacillus - chemistry
Infrared Rays
Lasers
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet
Spores, Bacterial - chemistry
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Zusammenfassung:The performance of infrared (2.94 microm) and ultraviolet (337 nm) lasers were compared for analysis of purified spores of B. subtilis, B. cereus and B. globigii on a four-inch end-cap reflectron time-of-flight instrument. Infrared matrix-assisted laser desorption/ionization (IR-MALDI) mass spectra of these microorganisms displayed a larger number of biomarker peaks above m/z 4000, compared with UV-MALDI. Biomarker peaks were observed at higher m/z values with the IR laser.
ISSN:0951-4198