The role of osmotic resistance on equine spermatozoal function

The role of osmotic resistance on equine spermatozoal function

Cryopreservation requires exposure of sperm to extreme variations in temperature and osmolality. The goal of this experiment was to determine the osmotic tolerance levels of equine sperm by analyzing motility, viability, mitochondrial membrane potential (MMP), and mean cell volume (MCV). Spermatozoa...

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Veröffentlicht in:Theriogenology 2002-10, Vol.58 (7), p.1373-1384
Hauptverfasser: Pommer, Angela C, Rutllant, Josep, Meyers, Stuart A
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Membrane - physiology
Cryopreservation - methods
Cryopreservation - veterinary
Female
Horses - physiology
Male
Membrane Potentials - physiology
Mitochondria - physiology
Mitochondrial membrane potential
Motility
Osmolar Concentration
Osmotic tolerance
Semen Preservation - methods
Semen Preservation - veterinary
Sperm function
Sperm Motility
Spermatozoa - physiology
Stallion
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Zusammenfassung:Cryopreservation requires exposure of sperm to extreme variations in temperature and osmolality. The goal of this experiment was to determine the osmotic tolerance levels of equine sperm by analyzing motility, viability, mitochondrial membrane potential (MMP), and mean cell volume (MCV). Spermatozoa were incubated at 22 °C for 10 min in isosmolal TALP (300 mOsm/kg), or a range of anisosmolal TALP solutions (75–900 mOsm/kg), for initial analysis, and then returned to isosmolal conditions for 10 min for further analysis. Total sperm motility was lower ( P
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(02)01039-7