Role of Endogenous Nitric Oxide Generation in the Regulation of Vascular Tone and Reactivity in Small Vessels as Investigated in Transgenic Mice Using Synchrotron Radiation Microangiography
Nitric oxide (NO) produced by endothelial NO synthase (eNOS) plays a central role in regulation of vascular tone and reactivity. The purpose of this study is to clarify the basal tone and microvascular reactivity in eNOS-overexpressing transgenic (Tg) mice in vivo with a microangiography system usin...
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| Veröffentlicht in: | Nitric oxide 2001-10, Vol.5 (5), p.494-503 |
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| creator | Yamashita, Tomoya Kawashima, Seinosuke Ozaki, Masanori Namiki, Masayuki Satomi-Kobayashi, Seimi Seno, Tadashi Matsuda, Yasuaki Inoue, Nobutaka Hirata, Ken-ichi Akita, Hozuka Umetani, Keiji Tanaka, Etsuro Mori, Hidezo Yokoyama, Mitsuhiro |
| description | Nitric oxide (NO) produced by endothelial NO synthase (eNOS) plays a central role in regulation of vascular tone and reactivity. The purpose of this study is to clarify the basal tone and microvascular reactivity in eNOS-overexpressing transgenic (Tg) mice in vivo with a microangiography system using monochromatic synchrotron radiation (SR). The mouse femoral artery was cannulated, nonionic contrast media was injected, and microangiography was performed in hindlimbs of mice. Serial images of the small blood vessels (diameter |
| doi_str_mv | 10.1006/niox.2001.0376 |
| format | Article |
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The purpose of this study is to clarify the basal tone and microvascular reactivity in eNOS-overexpressing transgenic (Tg) mice in vivo with a microangiography system using monochromatic synchrotron radiation (SR). The mouse femoral artery was cannulated, nonionic contrast media was injected, and microangiography was performed in hindlimbs of mice. Serial images of the small blood vessels (diameter <200 μm) were recorded by the SR microangiography system. At basal conditions, the diameter of tibial arteries in eNOS-Tg mice was larger than that of wild-type mice (179 ± 8 versus 132 ± 8 μm; P < 0.01). l-NAME treatment decreased the vessel diameter and canceled the difference in vessel diameters between two genotypes. Acetylcholine- and sodium nitroprusside-induced relaxations of small vessels were significantly reduced in Tg mice compared with wild-type mice (35.0 ± 9.4 versus 61.6 ± 6.7%, 85.0 ± 10.2 versus 97.3 ± 6.7% of the maximum relaxation, respectively). Our data provide the evidence that overproduced NO from endothelium reduces vascular tone and plays a pivotal role in regulation of vascular tone in small vessels. Furthermore, the reduced NO-mediated relaxation in small vessels of eNOS-Tg mice is demonstrated for the first time in vivo. SR microangiography allows us to evaluate the reactivity in small-sized vessels and appears to be a powerful tool for assessing the microvascular circulation in vivo.</description><identifier>ISSN: 1089-8603</identifier><identifier>EISSN: 1089-8611</identifier><identifier>DOI: 10.1006/niox.2001.0376</identifier><identifier>PMID: 11587564</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Angiography - methods ; Animals ; Cattle ; endothelial nitric oxide synthase ; Enzyme Inhibitors - pharmacology ; Hindlimb - blood supply ; Hindlimb - diagnostic imaging ; In Vitro Techniques ; Lipopolysaccharides - metabolism ; Mice ; Mice, Transgenic ; microangiography ; microvascular reactivity ; Muscle Contraction - drug effects ; Muscle Contraction - physiology ; Muscle Relaxation - drug effects ; Muscle Relaxation - physiology ; Muscle, Smooth, Vascular - physiology ; Neovascularization, Physiologic ; NG-Nitroarginine Methyl Ester - pharmacology ; nitric oxide ; Nitric Oxide - physiology ; Nitric Oxide Synthase - physiology ; Nitric Oxide Synthase Type II ; Nitric Oxide Synthase Type III ; Nitroprusside - pharmacology ; Papaverine - pharmacology ; synchrotron radiation ; Synchrotrons - instrumentation ; transgenic mouse ; vascular tone ; Vasodilation - drug effects ; Vasodilator Agents - pharmacology</subject><ispartof>Nitric oxide, 2001-10, Vol.5 (5), p.494-503</ispartof><rights>2001 Academic Press</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-4a345e9ce6fcb211f249dbff5ef6c96b6a405130e3e65b7fe13e0b581a030ec43</citedby><cites>FETCH-LOGICAL-c406t-4a345e9ce6fcb211f249dbff5ef6c96b6a405130e3e65b7fe13e0b581a030ec43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1089860301903763$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11587564$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamashita, Tomoya</creatorcontrib><creatorcontrib>Kawashima, Seinosuke</creatorcontrib><creatorcontrib>Ozaki, Masanori</creatorcontrib><creatorcontrib>Namiki, Masayuki</creatorcontrib><creatorcontrib>Satomi-Kobayashi, Seimi</creatorcontrib><creatorcontrib>Seno, Tadashi</creatorcontrib><creatorcontrib>Matsuda, Yasuaki</creatorcontrib><creatorcontrib>Inoue, Nobutaka</creatorcontrib><creatorcontrib>Hirata, Ken-ichi</creatorcontrib><creatorcontrib>Akita, Hozuka</creatorcontrib><creatorcontrib>Umetani, Keiji</creatorcontrib><creatorcontrib>Tanaka, Etsuro</creatorcontrib><creatorcontrib>Mori, Hidezo</creatorcontrib><creatorcontrib>Yokoyama, Mitsuhiro</creatorcontrib><title>Role of Endogenous Nitric Oxide Generation in the Regulation of Vascular Tone and Reactivity in Small Vessels as Investigated in Transgenic Mice Using Synchrotron Radiation Microangiography</title><title>Nitric oxide</title><addtitle>Nitric Oxide</addtitle><description>Nitric oxide (NO) produced by endothelial NO synthase (eNOS) plays a central role in regulation of vascular tone and reactivity. The purpose of this study is to clarify the basal tone and microvascular reactivity in eNOS-overexpressing transgenic (Tg) mice in vivo with a microangiography system using monochromatic synchrotron radiation (SR). The mouse femoral artery was cannulated, nonionic contrast media was injected, and microangiography was performed in hindlimbs of mice. Serial images of the small blood vessels (diameter <200 μm) were recorded by the SR microangiography system. At basal conditions, the diameter of tibial arteries in eNOS-Tg mice was larger than that of wild-type mice (179 ± 8 versus 132 ± 8 μm; P < 0.01). l-NAME treatment decreased the vessel diameter and canceled the difference in vessel diameters between two genotypes. Acetylcholine- and sodium nitroprusside-induced relaxations of small vessels were significantly reduced in Tg mice compared with wild-type mice (35.0 ± 9.4 versus 61.6 ± 6.7%, 85.0 ± 10.2 versus 97.3 ± 6.7% of the maximum relaxation, respectively). Our data provide the evidence that overproduced NO from endothelium reduces vascular tone and plays a pivotal role in regulation of vascular tone in small vessels. Furthermore, the reduced NO-mediated relaxation in small vessels of eNOS-Tg mice is demonstrated for the first time in vivo. SR microangiography allows us to evaluate the reactivity in small-sized vessels and appears to be a powerful tool for assessing the microvascular circulation in vivo.</description><subject>Angiography - methods</subject><subject>Animals</subject><subject>Cattle</subject><subject>endothelial nitric oxide synthase</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Hindlimb - blood supply</subject><subject>Hindlimb - diagnostic imaging</subject><subject>In Vitro Techniques</subject><subject>Lipopolysaccharides - metabolism</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>microangiography</subject><subject>microvascular reactivity</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Contraction - physiology</subject><subject>Muscle Relaxation - drug effects</subject><subject>Muscle Relaxation - physiology</subject><subject>Muscle, Smooth, Vascular - physiology</subject><subject>Neovascularization, Physiologic</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>nitric oxide</subject><subject>Nitric Oxide - physiology</subject><subject>Nitric Oxide Synthase - physiology</subject><subject>Nitric Oxide Synthase Type II</subject><subject>Nitric Oxide Synthase Type III</subject><subject>Nitroprusside - pharmacology</subject><subject>Papaverine - pharmacology</subject><subject>synchrotron radiation</subject><subject>Synchrotrons - instrumentation</subject><subject>transgenic mouse</subject><subject>vascular tone</subject><subject>Vasodilation - drug effects</subject><subject>Vasodilator Agents - pharmacology</subject><issn>1089-8603</issn><issn>1089-8611</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU9vGyEQxVHVqknTXnusOPVmF_YPu3usoiSNlDaS4-SKWBjWU63BBWzFH67frazWak45AcNv3hvNI-QzZ0vOmPjm0D8vC8b4kpWNeEPOOWu7RSs4f_v_zsoz8iHG34yxqmzFe3LGed02tajOyd-VH4F6S6-c8QM4v4_0F6aAmt4_owF6Aw6CSugdRUfTBugKhv04V3Lfk4o6PwNdewdUOZP_lU54wHScOh62ahzpE8QIY6Qq0lt3gJhwUAnMBKyDcjE7Z8efqIE-RnQDfTg6vQk-heyyUgZnvwwEr9yAfghqtzl-JO-sGiN8Op0X5PH6an35Y3F3f3N7-f1uoSsm0qJSZVVDp0FY3Rec26LqTG9tDVboTvRCVazmJYMSRN03FngJrK9brlgu6qq8IF9n3V3wf_Z5fLnFqGEclYO8MdkUvGmLpsvgcgbznDEGsHIXcKvCUXImp8DkFJicApNTYLnhy0l532_BvOCnhDLQzkBeHxwQgowawWkwGEAnaTy-pv0PqBapww</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>Yamashita, Tomoya</creator><creator>Kawashima, Seinosuke</creator><creator>Ozaki, Masanori</creator><creator>Namiki, Masayuki</creator><creator>Satomi-Kobayashi, Seimi</creator><creator>Seno, Tadashi</creator><creator>Matsuda, Yasuaki</creator><creator>Inoue, Nobutaka</creator><creator>Hirata, Ken-ichi</creator><creator>Akita, Hozuka</creator><creator>Umetani, Keiji</creator><creator>Tanaka, Etsuro</creator><creator>Mori, Hidezo</creator><creator>Yokoyama, Mitsuhiro</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011001</creationdate><title>Role of Endogenous Nitric Oxide Generation in the Regulation of Vascular Tone and Reactivity in Small Vessels as Investigated in Transgenic Mice Using Synchrotron Radiation Microangiography</title><author>Yamashita, Tomoya ; Kawashima, Seinosuke ; Ozaki, Masanori ; Namiki, Masayuki ; Satomi-Kobayashi, Seimi ; Seno, Tadashi ; Matsuda, Yasuaki ; Inoue, Nobutaka ; Hirata, Ken-ichi ; Akita, Hozuka ; Umetani, Keiji ; Tanaka, Etsuro ; Mori, Hidezo ; Yokoyama, Mitsuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-4a345e9ce6fcb211f249dbff5ef6c96b6a405130e3e65b7fe13e0b581a030ec43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Angiography - methods</topic><topic>Animals</topic><topic>Cattle</topic><topic>endothelial nitric oxide synthase</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Hindlimb - blood supply</topic><topic>Hindlimb - diagnostic imaging</topic><topic>In Vitro Techniques</topic><topic>Lipopolysaccharides - metabolism</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>microangiography</topic><topic>microvascular reactivity</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle Contraction - physiology</topic><topic>Muscle Relaxation - drug effects</topic><topic>Muscle Relaxation - physiology</topic><topic>Muscle, Smooth, Vascular - physiology</topic><topic>Neovascularization, Physiologic</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>nitric oxide</topic><topic>Nitric Oxide - physiology</topic><topic>Nitric Oxide Synthase - physiology</topic><topic>Nitric Oxide Synthase Type II</topic><topic>Nitric Oxide Synthase Type III</topic><topic>Nitroprusside - pharmacology</topic><topic>Papaverine - pharmacology</topic><topic>synchrotron radiation</topic><topic>Synchrotrons - instrumentation</topic><topic>transgenic mouse</topic><topic>vascular tone</topic><topic>Vasodilation - drug effects</topic><topic>Vasodilator Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamashita, Tomoya</creatorcontrib><creatorcontrib>Kawashima, Seinosuke</creatorcontrib><creatorcontrib>Ozaki, Masanori</creatorcontrib><creatorcontrib>Namiki, Masayuki</creatorcontrib><creatorcontrib>Satomi-Kobayashi, Seimi</creatorcontrib><creatorcontrib>Seno, Tadashi</creatorcontrib><creatorcontrib>Matsuda, Yasuaki</creatorcontrib><creatorcontrib>Inoue, Nobutaka</creatorcontrib><creatorcontrib>Hirata, Ken-ichi</creatorcontrib><creatorcontrib>Akita, Hozuka</creatorcontrib><creatorcontrib>Umetani, Keiji</creatorcontrib><creatorcontrib>Tanaka, Etsuro</creatorcontrib><creatorcontrib>Mori, Hidezo</creatorcontrib><creatorcontrib>Yokoyama, Mitsuhiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nitric oxide</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamashita, Tomoya</au><au>Kawashima, Seinosuke</au><au>Ozaki, Masanori</au><au>Namiki, Masayuki</au><au>Satomi-Kobayashi, Seimi</au><au>Seno, Tadashi</au><au>Matsuda, Yasuaki</au><au>Inoue, Nobutaka</au><au>Hirata, Ken-ichi</au><au>Akita, Hozuka</au><au>Umetani, Keiji</au><au>Tanaka, Etsuro</au><au>Mori, Hidezo</au><au>Yokoyama, Mitsuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Endogenous Nitric Oxide Generation in the Regulation of Vascular Tone and Reactivity in Small Vessels as Investigated in Transgenic Mice Using Synchrotron Radiation Microangiography</atitle><jtitle>Nitric oxide</jtitle><addtitle>Nitric Oxide</addtitle><date>2001-10-01</date><risdate>2001</risdate><volume>5</volume><issue>5</issue><spage>494</spage><epage>503</epage><pages>494-503</pages><issn>1089-8603</issn><eissn>1089-8611</eissn><abstract>Nitric oxide (NO) produced by endothelial NO synthase (eNOS) plays a central role in regulation of vascular tone and reactivity. The purpose of this study is to clarify the basal tone and microvascular reactivity in eNOS-overexpressing transgenic (Tg) mice in vivo with a microangiography system using monochromatic synchrotron radiation (SR). The mouse femoral artery was cannulated, nonionic contrast media was injected, and microangiography was performed in hindlimbs of mice. Serial images of the small blood vessels (diameter <200 μm) were recorded by the SR microangiography system. At basal conditions, the diameter of tibial arteries in eNOS-Tg mice was larger than that of wild-type mice (179 ± 8 versus 132 ± 8 μm; P < 0.01). l-NAME treatment decreased the vessel diameter and canceled the difference in vessel diameters between two genotypes. Acetylcholine- and sodium nitroprusside-induced relaxations of small vessels were significantly reduced in Tg mice compared with wild-type mice (35.0 ± 9.4 versus 61.6 ± 6.7%, 85.0 ± 10.2 versus 97.3 ± 6.7% of the maximum relaxation, respectively). Our data provide the evidence that overproduced NO from endothelium reduces vascular tone and plays a pivotal role in regulation of vascular tone in small vessels. Furthermore, the reduced NO-mediated relaxation in small vessels of eNOS-Tg mice is demonstrated for the first time in vivo. SR microangiography allows us to evaluate the reactivity in small-sized vessels and appears to be a powerful tool for assessing the microvascular circulation in vivo.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11587564</pmid><doi>10.1006/niox.2001.0376</doi><tpages>10</tpages></addata></record> |
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| subjects | Angiography - methods Animals Cattle endothelial nitric oxide synthase Enzyme Inhibitors - pharmacology Hindlimb - blood supply Hindlimb - diagnostic imaging In Vitro Techniques Lipopolysaccharides - metabolism Mice Mice, Transgenic microangiography microvascular reactivity Muscle Contraction - drug effects Muscle Contraction - physiology Muscle Relaxation - drug effects Muscle Relaxation - physiology Muscle, Smooth, Vascular - physiology Neovascularization, Physiologic NG-Nitroarginine Methyl Ester - pharmacology nitric oxide Nitric Oxide - physiology Nitric Oxide Synthase - physiology Nitric Oxide Synthase Type II Nitric Oxide Synthase Type III Nitroprusside - pharmacology Papaverine - pharmacology synchrotron radiation Synchrotrons - instrumentation transgenic mouse vascular tone Vasodilation - drug effects Vasodilator Agents - pharmacology |
| title | Role of Endogenous Nitric Oxide Generation in the Regulation of Vascular Tone and Reactivity in Small Vessels as Investigated in Transgenic Mice Using Synchrotron Radiation Microangiography |
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