Functional expression of Acetabularia acetabulum vacuolar H+‐pyrophosphatase in a yeast VMA3‐deficient strain

The function of the translation product of cDNA for Acetabularia vacuolar H+‐pyrophosphatase was examined using the Saccharomyces cerevisiae VMA3‐deficient strain. The open reading frame of Acetabularia H+‐pyrophosphatase was revealed to encode 751 amino acids (721 or 751 amino acids in a previous p...

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Veröffentlicht in:	Journal of experimental botany 2002-11, Vol.53 (378), p.2273-2275
Hauptverfasser:	Ikeda, Mikiko, Umami, Kimiko, Hinohara, Misato, Tanimura, Yoshie, Ohmae, Atsuko, Nakanishi, Yoichi, Maeshima, Masayoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetabularia - enzymology Acetabularia - genetics Amino acids Biochemistry Biological and medical sciences Cell physiology Complementary DNA Fundamental and applied biological sciences. Psychology Gels Gene Expression Regulation, Enzymologic GENE NOTE Genes. Genome heterologous expression Hydrogen-Ion Concentration Inorganic Pyrophosphatase Key words: Acetabularia acetabulum Microscopy, Fluorescence Molecular and cellular biology Molecular genetics Mutation Open reading frames Pelvic bones Plant physiology and development Plants Plasma membrane and permeation proton transport Pyrophosphatases - genetics Pyrophosphatases - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics vacuolar H+‐pyrophosphatase Vacuoles Vacuoles - enzymology Vacuoles - genetics yeast Yeasts
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