Functional expression of Acetabularia acetabulum vacuolar H+‐pyrophosphatase in a yeast VMA3‐deficient strain

The function of the translation product of cDNA for Acetabularia vacuolar H+‐pyrophosphatase was examined using the Saccharomyces cerevisiae VMA3‐deficient strain. The open reading frame of Acetabularia H+‐pyrophosphatase was revealed to encode 751 amino acids (721 or 751 amino acids in a previous p...

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Veröffentlicht in:	Journal of experimental botany 2002-11, Vol.53 (378), p.2273-2275
Hauptverfasser:	Ikeda, Mikiko, Umami, Kimiko, Hinohara, Misato, Tanimura, Yoshie, Ohmae, Atsuko, Nakanishi, Yoichi, Maeshima, Masayoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetabularia - enzymology Acetabularia - genetics Amino acids Biochemistry Biological and medical sciences Cell physiology Complementary DNA Fundamental and applied biological sciences. Psychology Gels Gene Expression Regulation, Enzymologic GENE NOTE Genes. Genome heterologous expression Hydrogen-Ion Concentration Inorganic Pyrophosphatase Key words: Acetabularia acetabulum Microscopy, Fluorescence Molecular and cellular biology Molecular genetics Mutation Open reading frames Pelvic bones Plant physiology and development Plants Plasma membrane and permeation proton transport Pyrophosphatases - genetics Pyrophosphatases - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics vacuolar H+‐pyrophosphatase Vacuoles Vacuoles - enzymology Vacuoles - genetics yeast Yeasts
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Zusammenfassung:	The function of the translation product of cDNA for Acetabularia vacuolar H+‐pyrophosphatase was examined using the Saccharomyces cerevisiae VMA3‐deficient strain. The open reading frame of Acetabularia H+‐pyrophosphatase was revealed to encode 751 amino acids (721 or 751 amino acids in a previous paper). The acidification of the vacuole was observed by fluorescence microscopy when the cDNA was constructed in pYES2. Immunoblot analysis also supported the localization of the translation product in the vacuolar‐membrane‐enriched fraction.
ISSN:	0022-0957 1460-2431 1460-2431
DOI:	10.1093/jxb/erf074