Triterpenoids from the leaves of Psidium guajava

Two triterpenoids, 20β-acetoxy-2α,3β-dihydroxyurs-12-en-28-oic acid (guavanoic acid, 3), and 2α,3β-dihydroxy-24- p- z-coumaroyloxyurs-12-en-28-oic acid (guavacoumaric acid, 7), along with six known compounds 2α-hydroxyursolic acid ( 1), jacoumaric acid ( 2), isoneriucoumaric acid ( 4), asiatic acid ( 5), ilelatifol D ( 6) and β-sitosterol-3- O-β- d-glucopyranoside ( 8), have been isolated from the leaves of Psidium guajava. Their structures were determined through spectroscopic methods. Compound 5 showed dose-dependent (10–500 μg/ml) spasmolytic activity in spontaneously contracting isolated rabbit jejunum preparations. Two triterpenoids, guavanoic acid ( 3) and guavacoumaric acid ( 7) along with six known compounds have been isolated from the leaves of Psidium guajava. Among them asiatic acid ( 5) showed dose-dependent (10–500 μ/ml) spasmolytic activity.