Xylitol production by recombinant Saccharomyces cerevisiae

We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR mono...

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Veröffentlicht in:	Bio/Technology 1991-11, Vol.9 (11), p.1090-1095
Hauptverfasser:	Hallborn, J. (University of Lund, Lund, Sweden), Walfridsson, M, Airaksinen, U, Ojamo, H, Hahn-Hagerdal, B, Penttila, M, Keranen, S
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Agriculture Aldehyde Reductase - biosynthesis Aldehyde Reductase - genetics Aldehyde Reductase - metabolism Amino Acid Sequence AMINO ACID SEQUENCES Animals Base Sequence Bioconversions. Hemisynthesis Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology CARBOHYDRATE METABOLISM Cloning, Molecular - methods ENZYMIC ACTIVITY Fundamental and applied biological sciences. Psychology GENE Gene Expression Gene Library GENES GENETIC TRANSFORMATION Humans Kinetics Life Sciences METABOLISME DES GLUCIDES METABOLISMO DE CARBOHIDRATOS Methods. Procedures. Technologies Molecular Sequence Data Molecular Weight NUCLEOTIDE NUCLEOTIDES NUCLEOTIDOS OXIDOREDUCTASES OXIDORREDUCTASAS OXYDOREDUCTASE PICHIA Pichia - enzymology Pichia - genetics PICHIA STIPITIS Recombinant Proteins - biosynthesis Recombinant Proteins - metabolism research-paper SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics Sequence Homology, Nucleic Acid TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE XILITOL XILOSA xyl1 gene XYLITOL Xylitol - metabolism XYLOSE Xylose - metabolism xylose reductase
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Zusammenfassung:	We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35,985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.
ISSN:	0733-222X 2331-3684 1546-1696
DOI:	10.1038/nbt1191-1090